利用高效液相色谱和紫外分析监测儿茶素酯化过程中的含量变化研究

儿茶素是一种广泛存在于植物体中的天然药物。现代科学研究表明儿茶素具有防治心血管疾病、预防癌症等多种功能。通过对儿茶素的酯化修饰,在分子中引入长碳链从而增加它的脂溶性及亲疏平衡性。本研究建立HPLC测定儿茶素含量的方法,色谱柱：Agi-lentZorbaxSB-C18（416×250 mm,5μm）,流动相：甲醇-水-冰醋酸（15∶84∶1）,流速：0.9 ml/min,检测波长：278 nm,测得儿茶素吸收峰的保留时间t=22 min左右,液相标准曲线为y=6×10^8x＋248 469,R^2=0.998 2,线性范围为0.003 24～0.006 43 mg;建立紫外分光光度计测定的方法：选用乙醇作为定容剂,特征峰为278 nm,测定儿茶素标准曲线为y=0.100 5x-0.040 1,R^2=0.999 4,线性范围为0.05～0.4 mg/ml。在儿茶素酯化过程的监控采用紫外可见分光光度计,方便快捷;利用HPLC可以监测酯化产物的生成状况,并可以准确计算合成收率。

Study on the Changes of the Catechins Content in the Esterification Process by Dint of HPLC and UV Methods

Catechins is widely present in plants in natural medicine.Modern scientific research shows that catechins can cure cardiovascular disease,cancer and other features.Through esterification,the introduction of the long carbon chain molecules can increase its hydrophilic and hydrophobic balance.This experiment established a HPLC method for the determination of catechins content.The column of AgilentZorbaxSB-C18(416 × 250 mm,5 μm) was used.The mobile phase was composed of formic acid-water-acetic acid(15∶84∶1) and the flow rate was 0.9 ml/min,with UV detection at 278 nm.Measured retention time of catechins absorption peak around t=22 min,HPLC standard curve y=6×108x+248 469,R2=0.998 2,linear range of 0.003 24-0.006 43 mg;UV spectrophotometer method was established:Ethanol as a constant volume solvent.The absorbance of catechins was measured at 278 nm.Determination of catechins standard curve was y=0.100 5x-0.040 1,R2= 0.999 4,linear range was 0.05-0.4 mg / ml.It is convenient to use UV-Vis spectrophotometer for the observation of catechins in the esterification.HPLC is applied to monitor the growth of esterification products and to calculate synthetic yield rate.