| 用16S rDNA序列分析方法鉴定4个拮抗细菌 |
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| 引用本文： | 王振军,刘玉霞,刘新涛,倪云霞,刘红彦. 用16S rDNA序列分析方法鉴定4个拮抗细菌[J]. 河南农业科学, 2006, 0(5): 59-61 |
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| 作者姓名： | 王振军 刘玉霞 刘新涛 倪云霞 刘红彦 |
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| 作者单位： | 1. 河南省农业科学院植物保护研究所,河南,郑州,450002;河南农业大学植物保护学院,河南,郑州,450002
2. 河南省农业科学院植物保护研究所,河南,郑州,450002 |
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| 基金项目： | 河南省财政预算外资金;河南省农业科学院基金 |
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| 摘 要： | 用1对16SrDNA通用引物，对4个拮抗细菌96-38、96-41、96-79和9682的基因组DNA进行扩增，PCR产物经克隆、测序、同源性分析表明，这4个菌株的16SrDNA全长均为1513bp，同源性达99．79％，仅在13个碱基位点存在差异。Blast分析发现，这些菌株与Genbank收录的芽孢杆菌属5个菌株16SrDNA序列的同源性为99．1％～99．9％，由此推断这4个拮抗细菌为芽孢杆菌属细菌。
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| 关 键 词： | 拮抗细菌 分子鉴定 |
| 文章编号： | 1004-3268(2006)05-0059-03 |
| 修稿时间： | 2006-02-17 |
Molecular Identification of Four Antagonistic Bacteria by Analyzing 16S rDNA Sequence |
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| WANG Zhen-jun,LIU Yu-xia,LIU Xin-tao,NI Yun-xia,LIU Hong-yan. Molecular Identification of Four Antagonistic Bacteria by Analyzing 16S rDNA Sequence[J]. Journal of Henan Agricultural Sciences, 2006, 0(5): 59-61 |
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| Authors: | WANG Zhen-jun LIU Yu-xia LIU Xin-tao NI Yun-xia LIU Hong-yan |
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| Abstract: | The genomic DNA samples from four strains of antagonistic bacteria were amplified with universal 16S rDNA primers.The products were cloned and sequenced.Homological analysis revealed that the sequences of four strains were all 1513bp with 99.79% identity and 13 base loci were different.Blast analysis showed that the 16S rDNA homology was 99.1% to 99.9% between the 4 tested stains and 5 stains of Bacillus in the GenBank.So it was concluded that the four antagonistic bacteria belonged to Bacillus. |
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| Keywords: | 16S rDNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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