| 实时定量PCR在植物真菌病原体定量检测中的应用 |
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| 引用本文： | 李凤兰,李学湛,闵凡祥,韩峰,魏琪,冯艳忠,郭梅. 实时定量PCR在植物真菌病原体定量检测中的应用[J]. 东北农业大学学报, 2010, 41(4) |
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| 作者姓名： | 李凤兰 李学湛 闵凡祥 韩峰 魏琪 冯艳忠 郭梅 |
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| 作者单位： | 东北农业大学生命科学学院,哈尔滨,150030;黑龙江省农业科学院植物脱毒苗木研究所,哈尔滨,150086;黑龙江省农业科学院植物脱毒苗木研究所,哈尔滨,150086;东北农业大学生命科学学院,哈尔滨,150030;黑龙江省农业科学院畜牧研究所,哈尔滨,150010 |
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| 基金项目： | 马铃薯产业创新体系项目，黑龙江省博士后启动基金，东北农业大学科学研究基金 |
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| 摘 要： | 实时定量PCR(Real-time Quantitative PCR,RQ-PCR)凭借其特异性强、定量准确和速度快等优点已经成为快速检测和研究植物真菌病害的重要工具。文章就这项技术在植物真菌病原菌定性和定量检测、土壤栖息的植物真菌病原体和寄主组织中真菌DNA的定量检测等方面的应用研究进行了综述。大量研究表明,快速发展的实时定量PCR技术正在开创一个植物真菌病害的诊断学、传染病学和寄主-病原体相互作用研究的新领域。
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| 关 键 词： | 实时定量PCR 定性和定量检测 植物真菌病害 土壤栖息真菌 |
Application of real-time quantitative PCR on detection of fungal plant disease |
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| LI Fenglan,LI Xuezhan,MIN Fanxiang,HAN Feng,WEI Qi,FENG Yanzhong,GUO Mei. Application of real-time quantitative PCR on detection of fungal plant disease[J]. Journal of Northeast Agricultural University, 2010, 41(4) |
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| Authors: | LI Fenglan LI Xuezhan MIN Fanxiang HAN Feng WEI Qi FENG Yanzhong GUO Mei |
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| Abstract: | Real-time Quantitative PCR(RQ-PCR)has become an important tool for rapidly detecting and studying fungal plant disease depending on its high specificity,exact quantitation and fast speed.The article reviewed this technology application in detection of fungal plant disease,including pathogenic bactedum qualitative and quantitative detection of fungal plant disease,fungal DNA quantification in host tissue and soil-inhabiting fungi.These studies showed that rapid growth RQ-PCR was opening a new research opportunity for the study of diagnosis,epidemiology and host-pathogen interactions. |
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| Keywords: | real-time quantitative PCR qualitative and quantitative detection fungal plant disease soil-inhabiting fungi |
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