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铁钉菜乙醇提取物对真菌生长的影响
引用本文:欧阳桐娇,郑怡,刘艳如,林勇,郭文杰,江枝和.铁钉菜乙醇提取物对真菌生长的影响[J].江西农业学报,2007,19(11):70-72.
作者姓名:欧阳桐娇  郑怡  刘艳如  林勇  郭文杰  江枝和
作者单位:1. 福建省农科院,生物技术研究所,福建,福州,350003
2. 福建师范大学,生命科学院,福建,福州,350007
3. 福建省农科院,土壤肥料研究所,福建,福州,350013
基金项目:福建省科技厅重点项目(2005N036)
摘    要:铁钉菜乙醇粗提物通过薄层层析法分离活性物质,分别采用纸片法和菌饼法测定了铁钉菜提取物及薄层层析带对3种真菌的菌丝生长的影响,用玻片法测定了对孢子萌发的抑制作用。实验结果表明:乙醇提取物对3种真菌孢子萌发的抑制作用最强,其中,浓度在0.005 mg/mL时对绿色木霉和黑曲霉孢子萌发的抑制率均达到99%。

关 键 词:铁钉菜  薄层层析  真菌  菌丝生长  孢子萌发
文章编号:1001-8581(2007)11-0070-03
收稿时间:2007-08-06
修稿时间:2007年8月6日

Effect of Ethanol Extract from Ishige okamura on Growth of Fungi
OUYANG Tong-jiao,ZHENG Yi,LIU Yan-ru,LIN Yong,GUO Wen-jie,JIANG Zhi-he.Effect of Ethanol Extract from Ishige okamura on Growth of Fungi[J].Acta Agriculturae Jiangxi,2007,19(11):70-72.
Authors:OUYANG Tong-jiao  ZHENG Yi  LIU Yan-ru  LIN Yong  GUO Wen-jie  JIANG Zhi-he
Institution:1. Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China; 2. College of Life Sciences, Fujian Normal University, Fuzhou 350007, China; 3. Institute of Soil and Fertilizer, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China
Abstract:Active substances separated by TLC from the ethanol extract of Ishige okamura were used to study their effects on the hyphal growth of three fungi by using the method of disc and filter paper,as well as their restraining effects on the spore germination by using the slide method.The results indicated that ethanol extract showed strong inhibition to the spore germination of three fungi and the maximum inhibitory rate reached 97.0% to Trichoderma viride and Aspergillus niger under the concentration of 0.005 mg/mL.
Keywords:Ishige okamura  Thin layer chromatographic analysis  Fungus  Hyphal growth  Spore germination
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