牛催乳素受体(PRLR)基因HinfⅠ多态性与奶牛生产性能相关性研究

采用PCR-RFLP方法检测了牛催乳素受体（PRLR）基因intron 8多态性。结果表明：中国荷斯坦牛、加拿大荷斯坦和澳洲荷斯坦牛群体中HinfⅠ酶切后均有A和B两个等位基因存在。利用最小二乘分析研究了该多态位点对奶牛305 d产奶成年当量、乳脂肪率、乳蛋白率、初次配种日龄和初次产犊日龄的影响。结果表明：不同基因型个体的乳脂率、初次配种日龄和初次产犊日龄的差异均未达到显著水平,但呈现出AA〉AB〉BB的趋势。BB型个体的305 d成年当量与AA型、AB型个体有显著差异（P〈0.05）,BB型个体的乳蛋白率与AA型、AB型个体有极显著差异（P〈0.01）。对305 d成年当量、乳脂肪率、乳蛋白率、初次配种日龄和初次产犊日龄A基因的加性效应分别为296.00 kg/胎次、0.08%-、0.14%、4.55 d/头、15.10 d/头,A等位基因的显性效应为259.80 kg/胎次、0.07%、-0.10%、0.95 d/头和4.90 d/头。

Association between Hinf Ⅰ Polymorphism of Prolactin Receptor (PRLR) Gene and Performance of Dairy Cows

The polymorphism of intron 8 in prolactin receptor(PRLR) gene was detected by PCR-RFLP.A Hinf-I RFLP was detected in intron 8 and the polymorphic locus is common in all detected lines.Association between the founded Hinf-I RFLP in intron 8 and 305 days matured equivalency(ME),fat percent(F%),protein percent(P%),the first mating days(FMD) and the first calving days(FCD) was tested by least square analysis.No significant difference between Hinf-I RFLP and F%,FMD and FCD were founded in present study,but regular effects was showed.Individuals with BB genotype had lower ME(P<0.05) and higher P%(P<0.01).The additive effects of allele A was 296.00 kg/parity,0.08,-0.14%,4.55 d/cow,15.10 d/cow for ME,F%,P%,FMD and FCD respectively.The dominant effects of allele A was 259.80 kg/parity,0.07,-0.10%,0.95 d/cow and 4.90 d/cow respectively.