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Genetic variability among sugarcane genotypes based on polymorphisms in sucrose metabolism and drought tolerance genes
Authors:Silvana Creste  Klaus A G Accoroni  Luciana R Pinto  Roland Vencovsky  Marcos A Gimenes  Mauro A Xavier  Marcos G A Landell
Institution:1.Centro de Cana,Instituto Agron?mico de Campinas,Ribeir?o Preto,Brazil;2.Departamento de Genética, Escola Superior de Agricultura “Luiz de Queiroz”,Universidade de S?o Paulo,Piracicaba,Brazil;3.Embrapa Recursos Genéticos e Biotecnologia (CENARGEN),Brasília,Brazil
Abstract:Target region amplification polymorphism (TRAP) markers were used to estimate the genetic similarity (GS) among 53 sugarcane varieties and five species of the Saccharum complex. Seven fixed primers designed from candidate genes involved in sucrose metabolism and three from those involved in drought response metabolism were used in combination with three arbitrary primers. The clustering of the genotypes for sucrose metabolism and drought response were similar, but the GS based on Jaccard’s coefficient changed. The GS based on polymorphism in sucrose genes estimated in a set of 46 Brazilian varieties, all of which belong to the three Brazilian breeding programs, ranged from 0.52 to 0.9, and that based on drought data ranged from 0.44 to 0.95. The results suggest that genetic variability in the evaluated genes was lower in the sucrose metabolism genes than in the drought response metabolism ones.
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