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Construction of a linkage map for watermelon (Citrullus lanatus (Thunb.) Matsum & Nakai) using random amplified polymorphic DNA (RAPD)
Authors:Toshiharu Hashizume  Ikuhiro Shimamoto  Yoshiaki Harushima  Mamiko Yui  Takanori Sato  Tsuyoshi Imai  Masashi Hirai
Institution:(1) Hagihara Farm Production Institute Co. Ltd., Hokiji, 636-03 Tawaramoto, Japan;(2) Rice Genome Research Program, National Institute of Agrobiological Resources (NIAR)/Society for Techno-innovation of Agriculture, Foresty and Fisheries (STAFF), 305 Tsukuba, Japan;(3) Department of Vegetable Breeding, National Institute of Vegetable Ornamental Plants and Tea (NIVOT) Ano, 514-23, Japan
Abstract:Summary A linkage map for watermelon (Citrullus lanatus) was constructed on the basis of RADP, ribosomal DNA restriction fragment length polymorphism (RFLP), isozyme, and morphological markers using F1BC1. A segregating population of 78 individuals was the result of a backcross of a cultivated inbred line (H-7; Citrullus lanatus; 2n=22) and a wild form (SA-1; C. lanatus; 2n=22), in which the latter was the recurrent (male) parent. A total of 69 RAPD, one RFLP, one isozyme, and three morphological markers was found to segregate in the BC1 population. Linkage analysis revealed that 62 loci could be mapped to 11 linkage groups that extended more than 524 centimorgans (cM), while 12 loci segregated independently of all other markers. The locus for exocarp color was linked to two RAPD markers within a region of 5 cM on linkage group 4. The locus for flesh color was linked to a RAPD marker within a region of 30 cM on linkage group 6. The isozyme marker GOT was located on the linkage group 1. Linkage group 2 contained a locus for ribosomal DNA within 5 cM of a RAPD marker. Half of the RAPD markers on the linkage group 7 displayed severely distorted segregation. The construction of linkage map using molecular markers is necessary for the breeding of watermelon to introduce useful gene of wild watermelon efficiently. However the linkage map that was constructed for the most part on the basis of RAPD markers could not cover significant parts of the genome, the linkage map provides breeders of watermelons the possibility of tagging useful agronomic traits, as well as the gene for exocarp color.Abbreviations RAPD random amplified polymorphic DNA - RFLP restriction fragment length polymorphism - GOT glutamate oxaloacetate transaminase - MDH malate dehydrogenase - ACP acid phosphatase - 6PGH 6-phosphogluconate dehydrogenase
Keywords:Citrullus lanatus  RAPD  linkage map  genetic marker  watermelon  exocarp color  flesh color
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