Construction of a linkage map for watermelon (Citrullus lanatus (Thunb.) Matsum & Nakai) using random amplified polymorphic DNA (RAPD) |
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Authors: | Toshiharu Hashizume Ikuhiro Shimamoto Yoshiaki Harushima Mamiko Yui Takanori Sato Tsuyoshi Imai Masashi Hirai |
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Institution: | (1) Hagihara Farm Production Institute Co. Ltd., Hokiji, 636-03 Tawaramoto, Japan;(2) Rice Genome Research Program, National Institute of Agrobiological Resources (NIAR)/Society for Techno-innovation of Agriculture, Foresty and Fisheries (STAFF), 305 Tsukuba, Japan;(3) Department of Vegetable Breeding, National Institute of Vegetable Ornamental Plants and Tea (NIVOT) Ano, 514-23, Japan |
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Abstract: | Summary A linkage map for watermelon (Citrullus lanatus) was constructed on the basis of RADP, ribosomal DNA restriction fragment length polymorphism (RFLP), isozyme, and morphological markers using F1BC1. A segregating population of 78 individuals was the result of a backcross of a cultivated inbred line (H-7; Citrullus lanatus; 2n=22) and a wild form (SA-1; C. lanatus; 2n=22), in which the latter was the recurrent (male) parent. A total of 69 RAPD, one RFLP, one isozyme, and three morphological markers was found to segregate in the BC1 population. Linkage analysis revealed that 62 loci could be mapped to 11 linkage groups that extended more than 524 centimorgans (cM), while 12 loci segregated independently of all other markers. The locus for exocarp color was linked to two RAPD markers within a region of 5 cM on linkage group 4. The locus for flesh color was linked to a RAPD marker within a region of 30 cM on linkage group 6. The isozyme marker GOT was located on the linkage group 1. Linkage group 2 contained a locus for ribosomal DNA within 5 cM of a RAPD marker. Half of the RAPD markers on the linkage group 7 displayed severely distorted segregation. The construction of linkage map using molecular markers is necessary for the breeding of watermelon to introduce useful gene of wild watermelon efficiently. However the linkage map that was constructed for the most part on the basis of RAPD markers could not cover significant parts of the genome, the linkage map provides breeders of watermelons the possibility of tagging useful agronomic traits, as well as the gene for exocarp color.Abbreviations RAPD
random amplified polymorphic DNA
- RFLP
restriction fragment length polymorphism
- GOT
glutamate oxaloacetate transaminase
- MDH
malate dehydrogenase
- ACP
acid phosphatase
- 6PGH
6-phosphogluconate dehydrogenase |
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Keywords: | Citrullus lanatus RAPD linkage map genetic marker watermelon exocarp color flesh color |
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