| 新西伯利亚银白杨组织培养基配方的筛选与应用 |
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| 引用本文： | 史绍林,吴长江,赵凌泉,徐连峰. 新西伯利亚银白杨组织培养基配方的筛选与应用[J]. 防护林科技, 2008, 0(6): 25-26 |
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| 作者姓名： | 史绍林 吴长江 赵凌泉 徐连峰 |
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| 作者单位： | 黑龙江省森林与环境科学研究院,黑龙江齐齐哈尔,161005 |
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| 基金项目： | 黑龙江省科技攻关项目 |
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| 摘 要： | 以新西伯利亚银白杨叶片为外植体，建立了再生体系。经正交试验筛选出此组织培养的最适外植体产生不定芽诱导培养基为MS＋6-BA0．1mg·L^-1＋GA0．1mg·L^-1＋NAA0．05mg·L^-1，最佳不定芽生根培养基为1／2MS＋NAA0．1mg·L^-1‘＋IBA0．02mg·L^-1。
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| 关 键 词： | 新西伯利亚银白杨 叶片外植体 再生体系 |
Screening and Application of Constitution of Medium for Populus hybride |
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| SHI Shao-lin,WU Chang-jiang,ZHAO Ling-quan,XU Lian-feng. Screening and Application of Constitution of Medium for Populus hybride[J]. Protection Forest Science and Technology, 2008, 0(6): 25-26 |
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| Authors: | SHI Shao-lin WU Chang-jiang ZHAO Ling-quan XU Lian-feng |
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| Affiliation: | SHI Shao-lin,WU Chang-jiang,ZHAO Ling-quan,XU Lian-feng(Academy of Forest , Environment of Heilongjiang Province,Qiqihar 161005,Heilongjiang,China) |
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| Abstract: | Leaves of Populus hybride were used as explants to set up regeneration system.The optimal medium formula(MS+ 6-BA 0.1 mg·L-1 + GA 0.1 mg·L-1 + NAA 0.05 mg·L-1) which is suitable to adventitious bud induction for explant was screened out by orthogonal test.The optimal medium of root regeneration from adventitious bud is 1/2 MS+ NAA 0.1 mg·L-1 +IBA 0.02 mg·L-1. |
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| Keywords: | Populus hybride leaf explants regeneration system |
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