柱花草DUS测试标准品种DNA指纹图谱构建

为建立柱花草(Stylosanthes spp.)DUS测试标准品种DNA指纹图谱数据库,实现柱花草品种的快速、准确鉴定,利用筛选到的25个SSR标记对15份柱花草DUS测试标准品种进行了遗传多样性分析及DNA指纹图谱构建。结果表明,25个SSR标记共产生132个等位基因,每个标记可产生3~9个等位基因,平均5.280个;各SSR标记的多态性信息含量为0.393~0.847,平均为0.638。利用这25个SSR标记构建的指纹图谱能有效区分15份柱花草DUS标准品种,并为每份标准品种建立了QR二维码图谱,为SSR标记技术在柱花草DUS测试中的应用提供依据。

Construction of DNA fingerprinting of Stylosanthes standard cultivars for DUS testing

DNA fingerprinting of Stylosanthes standard cultivars for distinctness, uniformity and stability (DUS) testing was constructed to facilitate the identification of Styosanthes cultivars. The genetic diversity analysis and DNA fingerprinting construction of 15 Stylosanthes standard cultivars for DUS testing were performed using 25 SSR markers. The results indicated that there were total of 132 alleles were generated from 25 SSR markers among these 15 standard cultivars. The number of alleles generated from each SSR marker ranged from 3~9, with an average of 5.280 alleles. The polymorphic information content per SSR marker varied from 0.393 to 0.847, with an average of 0.638. The 15 standard cultivars could be successfully distinguished by DNA fingerprinting, which was constructed based on these 25 SSR markers. The DNA fingerprinting QR code for each Stylosanthes standard cultivars was constructed. The results could provide important reference for application of SSR marker in Stylosanthes DUS testing.