| 潮间带菌株CZⅣ-1068的鉴定及抑菌活性研究 |
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| 引用本文： | 王健鑫,;刘雪珠,;李鹏,;陈小龙. 潮间带菌株CZⅣ-1068的鉴定及抑菌活性研究[J]. 浙江水产学院学报, 2008, 0(4): 401-405 |
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| 作者姓名： | 王健鑫, 刘雪珠, 李鹏, 陈小龙 |
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| 作者单位： | [1]浙江海洋学院海洋科学学院,浙江舟山316004; [2]浙江工业大学生物与环境工程学院,浙江杭州310032 |
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| 基金项目： | 浙江省教育厅计划项目（20061133）;舟山市科技局计划项目（06226） |
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| 摘 要： | CZⅣ-1068是从舟山长峙岛潮间带海泥中分离到的一株细菌,最适NaCl浓度范围为1%～3.5%,经琼脂扩散法抗菌模型筛选表明对大肠杆菌、副溶血性弧菌、枯草芽孢杆菌、金黄色葡萄球菌、藤黄微球菌和白假丝酵母有拮抗作用。利用形态学观察、生理生化反应对菌株进行了初步研究,并利用PCR的方法扩增了CZⅣ-1068的16S rDNA,然后进行核苷酸序列测定分析,结果表明CZⅣ-1068与Bacillus sp.82344（AF227848）相似性高达100%,初步确定为腊状芽孢杆菌的一种。
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| 关 键 词： | 潮间带 腊状芽孢杆菌 抑菌活性 鉴定 |
Identification and Antimicrobial Activity of Strain CZIV-1068 from Sediment of Intertidal Zone |
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| Affiliation: | WANG Jian-xin, LIU Xue-zhu, LI Peng, et al (1.Marine Science School of Zhejiang Ocean Environmental Engineering, Zhejiang University, Zhoushan 316004; 2. College of Biological and University of Technology, Hangzhou 310032, China) |
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| Abstract: | CZⅣ-1068, which was isolated from sediments of intertidal zone in Changzhi island, is weekly halophilic bacterium and its optimum range of salt concentration is 1%-3.5% of NaCl. It was found that the metabolities of the strain had antagonism on Escherichia coli, Vibrio parahaemolyticus, Bacillus subtilis, Staphylococcus aureus, Micrococcus luteus and Candida albicans by using agar diffusion method. CZⅣ-1068 was classified into Bacillus cereus subgroup based on morphology, analysis of physiology and biochemistry and molecular biology methods. The sequence of 16S rDNA from CZⅣ-1068 strain was similar to Bacillus sp. 82344 （AF227848） compared with those in database. The evolutional distance was calculated and phylogenetic tree was drawn based on the sequence data. |
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| Keywords: | intertidal zone Bacillus cereus antimicrobial activity identification |
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