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猪O型口蹄疫免疫抗体检测方法的比较
引用本文:张玉,;周金梅,;李丽丽,;魏巍,;杨继元. 猪O型口蹄疫免疫抗体检测方法的比较[J]. 内蒙古畜牧科学, 2014, 0(4): 117-119
作者姓名:张玉,  周金梅,  李丽丽,  魏巍,  杨继元
作者单位:[1]青海省大通县景阳镇畜牧兽医站,青海大通810100; [2]青海省大通县畜牧兽医站,青海大通810100
摘    要:试验采用口蹄疫O型间接血凝试验和O型口蹄疫抗体液相阻断ELISA 2种试验方法检测了60份血清中猪O型口蹄疫免疫抗体.研究结果显示,60份被检血清口蹄疫O型间接血凝试验检测合格率为93.3%,O型口蹄疫抗体液相阻断ELISA试验检测的合格率为73.3%,口蹄疫O型间接血凝试验检测合格率明显高于O型口蹄疫抗体液相阻断ELISA试验检测的合格率(相差20个百分点);2种方法的总符合率为66.7%、<25(<2^6)的符合率为28.6%,≥2^5(≥2^6)的符合率为82.1%.2种方法检测出的整体免疫效果较好,平均合格率远高于农业部规定的70%.

关 键 词:O型口蹄疫  免疫抗体  检测方法

Comparison of the Detection Methods of Immune Antibody of O-type Foot-and-mouth Disease of Pigs
Affiliation:ZHANG Yu, ZHOU Jin-mei, LI Li-li, WEI Wei, YANG Ji-yuan ( Animal Husbandry and Veterinary Station in Jingyang Town of Datong County, Qinghai Province, Datong 810100, China; Animal Husbandry and Veterinary Station in Datong County of Qinghai Province, Datong 810100, China)
Abstract:The immune antibody of O-type foot-and-mouth disease in 60 serum samples were detected by using two test methods of O-type foot-and-mouth disease indirect hemagglutination antibody and O-type foot-and-mouth disease antibody liquid-phase blocking ELISA. The results showed that the qualification rate of 60 serum samples in O-type foot-and-mouth disease indirect hemagglutination assay was 93.3% and that in O-type foot-and-mouth disease antibody liquid-phase blocking ELISA was 73.3%. The qualification rate in O-type foot-and-mouth disease indirect hemagglutination assay was obviously higher than that in O-type foot-and-mouth disease antibody liquid-phase blocking ELISA (higher 20% ). The whole coincidence rate of two methods was 66.7%, that of less than 25(less than 26) was 28.6% and that of ≥25( ≥26)was 82.1%. The whole immune effects of two detection methods were both better, and the average qualification rate was greatly higher than the specified value (70%) of Ministry of Agriculture.
Keywords:O-type foot-and-mouth disease  immune antibody  detection methods
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