| 紫茎泽兰抑制马铃薯晚疫病菌的初步研究 |
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| 引用本文： | 丁君辉,杨艳丽,朱菲莹,王若仲,萧浪涛. 紫茎泽兰抑制马铃薯晚疫病菌的初步研究[J]. 湖南农业大学学报(自然科学版), 2007, 33(6): 751-753 |
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| 作者姓名： | 丁君辉 杨艳丽 朱菲莹 王若仲 萧浪涛 |
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| 作者单位： | 湖南农业大学湖南省植物激素与生长发育重点实验室,湖南,长沙,410128;云南农业大学,植物保护学院,云南,昆明,650201;湖南农业大学生物科学技术学院,湖南,长沙,410128 |
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| 基金项目： | 湖南农业大学拔尖人才项目(BJ2004) |
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| 摘 要： | 将紫茎泽兰的乙醇提取物依次用石油醚、氯仿、乙酸乙酯、正丁醇萃取,萃取物对马铃薯晚疫病菌进行抑菌试验.结果表明,石油醚萃取物抑菌效果最好,抑菌率达100%.进一步采用不同梯度的混合有机溶剂对石油醚萃取物进行硅胶柱层析,抑菌试验结果表明,用石油醚:乙酸乙酯(8:1)、石油醚:乙酸乙酯(4:1)和甲醇试剂洗脱得到的有效物质,其抑菌效果最好,抑菌率达100%.
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| 关 键 词： | 紫茎泽兰 马铃薯晚疫病菌 抑菌 |
| 文章编号： | 1007-1032（2007）06-0751-03 |
| 收稿时间： | 2007-07-31 |
| 修稿时间： | 2007-07-31 |
On antibacterial activities of Eupatorium adenophorum |
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| DING Jun-hui,YANG Yan-li,ZHU Fei-ying,WANG Ruo-zhong,XIAO Lang-tao. On antibacterial activities of Eupatorium adenophorum[J]. Journal of Hunan Agricultural University, 2007, 33(6): 751-753 |
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| Authors: | DING Jun-hui YANG Yan-li ZHU Fei-ying WANG Ruo-zhong XIAO Lang-tao |
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| Abstract: | Extraction of Eupatorium adenophorum Spreng has the higher suppression effects on Phytophthora infestans (Mont.) de Bary.Corresponding extraction was obtained by extraction of Eupatorium adenophorum Spreng with petroleum ether,chloroform,ethyl acetate and normal butyl alcohol,followed by the subsequent steps: determination of the active ingredients of Eupatorium adenophorura Spreng derived from extraction of petroleum ether through the bacteriostasis,reuse of different of gradient mixed organic solvents to its concentration and the silica gel chromatographic analysis and ration collection.Through the bacteriostasis it is obviously revelaled that the centralized spot of the active ingredients of Eupatorium adenophorum Spreng,namely the elution of petroleum ether: ethyl acetate (8:1), the elution of petroleum ether:ethyl acetate(4:1). |
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| Keywords: | Eupatorium adenophorum Spreng Phytophthora infestans (Mont.) de Bary bacteriostasis |
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