| 龙山卷丹百合无症病毒的检测 |
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| 引用本文： | 蒋 辉,源朝政,陈海霞. 龙山卷丹百合无症病毒的检测[J]. 天津农业科学, 2014, 0(5): 27-30 |
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| 作者姓名： | 蒋 辉 源朝政 陈海霞 |
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| 作者单位： | 湖南农业大学园艺园林学院,湖南长沙410128 |
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| 基金项目： | 湖南省科技厅项目（2013NK3043）;湖南省自然基金（11JJ6022） |
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| 摘 要： | 以龙山卷丹百合的病叶作为试材,采用DAS-ELISAS检测法对样品进行百合无症病毒的检测,检出率为58.3%;提取总RNA为模板,通过RT-PCR扩增其外壳蛋白基因,大小为287 bp,和预期条带大小一致。经Blast比对发现,该基因片段与Genbank上发表的LSV CP基因序列同源性达92%以上。
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| 关 键 词： | 卷丹百合 RT-PCR DAS-ELISA 百合无症病毒 |
Detection of LSV Infecting Longshan Lilium tigrinum |
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| JIANG Hui,YUAN Chao-zheng,CHEN Hai-xia. Detection of LSV Infecting Longshan Lilium tigrinum[J]. Tianjin Agricultural Sciences, 2014, 0(5): 27-30 |
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| Authors: | JIANG Hui YUAN Chao-zheng CHEN Hai-xia |
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| Affiliation: | (College of Horticulture and Landscape, Hunan Agriculture University, Changsha, Hunan 410128, China) |
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| Abstract: | In this research, Lilium tigrinum of longshan was used as experimental material to be studied for virus detection. The result of DAS-ELISAS showed that the positive rate of LSV was 58.3%. Total RNA used as template, the coat protein gene by RT- PCR amplification size was 287 bp, the same as expected. By the Blast, the homology of this gene fragment and the LSV CP gene sequence that published in Genbank was more than 92%. |
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| Keywords: | Lilium tigrinum RT - PCR DAS - ELISA LSV |
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