| 实时荧光定量PCR技术在番茄研究中的应用 |
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| 引用本文： | 郝志愚,金凤媚,薛 俊,宋 建.实时荧光定量PCR技术在番茄研究中的应用[J].天津农业科学,2014(5):43-46. |
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| 作者姓名： | 郝志愚 金凤媚 薛 俊 宋 建 |
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| 作者单位： | 天津市农业生物技术研究中心,天津300384 |
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| 基金项目： | 天津市农业科学院院长基金项目（13014） |
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| 摘 要： | 随着实时荧光定量PCR技术的不断完善和发展,该技术越来越受到人们的青睐。它以快速、灵敏、高特异性和安全性高等优点广泛应用于科学研究等各个领域。近年来,实时荧光定量PCR技术在番茄的各研究领域不断深入,大大提高了番茄的病害检测能力、特异基因的表达水平和转基因的检测效率。
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| 关 键 词： | 实时荧光定量PCR 番茄 检测 |
Application of Real-time Fluorescence Quantitative PCR in Tomato |
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| HAO Zhi-yu,JIN Feng-mei,XUE Jun,SONG Jian.Application of Real-time Fluorescence Quantitative PCR in Tomato[J].Tianjin Agricultural Sciences,2014(5):43-46. |
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| Authors: | HAO Zhi-yu JIN Feng-mei XUE Jun SONG Jian |
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| Institution: | (Tianjin Research Center of Agricultural Biotechnology, Tianjin 300384, China) |
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| Abstract: | With the development of real-time Fluorescence Quantitative PCR technology, more attention was paid to the technology. It was a fast, sensitive and highly specific technology and have been used widely in various fields of scientific research. In recent years, real-time Fluorescence Quantitative PCR technology has been applied on the research of tomato. It greatly improved the detection of transgenic tomato , tomato disease and specific genes expression. |
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| Keywords: | real-time Fluorescence Quantitative PCR tomato determination |
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