| 精子受精抗原-1(FA-1) mRNA在绵羊睾丸和附睾中的表达 |
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| 引用本文： | 娜仁花,旭日干. 精子受精抗原-1(FA-1) mRNA在绵羊睾丸和附睾中的表达[J]. 畜牧与兽医, 2006, 38(11): 1-3 |
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| 作者姓名： | 娜仁花 旭日干 |
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| 作者单位： | 1. 内蒙古农业大学动物科学与医学学院,内蒙古,呼和浩特,010018;哺乳动物生殖生物学与生物技术教育部重点实验室,内蒙古大学生命科学学院实验动物研究中心,内蒙古,呼和浩特,010021
2. 哺乳动物生殖生物学与生物技术教育部重点实验室,内蒙古大学生命科学学院实验动物研究中心,内蒙古,呼和浩特,010021 |
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| 基金项目： | 国家自然科学基金(30560103)，内蒙古自然科学基金(200408020412)资助 |
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| 摘 要： | 本研究通过RT-PCR检测了该基因在绵羊睾丸和附睾中的表达情况。首先,提取绵羊睾丸组织、附睾头、体、尾部组织总RNA,以此为模板,反转录合成cDNA,自行设计引物,PCR扩增出462 bp的目的DNA。然后,将目的片断克隆入T载体,通过菌液PCR和重组质粒酶切,鉴定重组质粒中的目的DNA。再经序列分析鉴定目的片断。同时,以β-actin为内参照物,进行RT-PCR半定量分析,比较精子受精抗原(FA-1)在睾丸、附睾头、体、尾组织中的表达量。结果表明,FA-1在绵羊睾丸和附睾中均表达。
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| 关 键 词： | 精子受精抗原-1 RT-PCR T/A克隆 mRNA表达 |
| 文章编号： | 0529-5130（2006）11-0001-03 |
| 收稿时间： | 2006-03-06 |
| 修稿时间： | 2006-03-06 |
The expression of sperm fertilization antigen-1 (FA-1) mRNA in the testis and epididymis of ram |
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| NA Ren-hua,XU Ri-gan. The expression of sperm fertilization antigen-1 (FA-1) mRNA in the testis and epididymis of ram[J]. Animal Husbandry & Veterinary Medicine, 2006, 38(11): 1-3 |
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| Authors: | NA Ren-hua XU Ri-gan |
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| Affiliation: | 1. College of Animal Science and Medicine, Inner Mongolia Agricultural University, Huhhot 010018, China; 2. Key Laboratory of Mammal Reproductive Biology and Biotechnology Ministry of Education, The Research Center for Laboratory Animal Science of Life Science of College of Inner Mongolia University, Huhhot 010021, China |
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| Abstract: | Sperm fertilization antigen-1 plays a crucial role in mammalian fertilization and development of fertilized oocyte.In this study,total RNA was prepared from sheep testis and epididymis.cDNA fragment was amplified by RT-PCR using specific primers,and cloned into pGM-T Easy vector.Inserted FA-1 gene was sequenced.Meanwhile,semi-quantitative analysis of FA-1 expression in testis and caput,corpus,cauda epididymis was carried out by RT-PCR based on β-actin as an inner control.The results indicated that the FA-1 gene was expressed in both testis and epididymis of sheep. |
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| Keywords: | sperm fertilization antigen-1 RT-PCR T/A cloning mRNA expression |
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