| 水稻二化螟病原真菌鉴定及其致病力 |
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| 引用本文： | 李美君,白庆荣,臧连生,阮长春.水稻二化螟病原真菌鉴定及其致病力[J].中国生物防治学报,2019,35(1):63-69. |
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| 作者姓名： | 李美君 白庆荣 臧连生 阮长春 |
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| 作者单位： | 1. 吉林农业大学生物防治研究所/吉林省生物防治应用技术工程研究中心, 长春 130118;2. 吉林农业大学农学院, 长春 130118 |
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| 基金项目： | 国家重点研发计划（2017YFD0201200） |
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| 摘 要： | 通过对吉林本地自然越冬代罹病的水稻二化螟幼虫进行分离纯化，筛选出两个病原真菌JL003和JL005，采用形态特征比较和rDNA-ITS序列构建系统树进行分析，并采用浸渍法研究了2个菌株对二化螟3龄和6龄幼虫的毒力作用。鉴定结果表明，菌株JL003和JL005分别为渐狭蜡蚧菌和球孢白僵菌，其中，渐狭蜡蚧菌侵染水稻二化螟为国内新近报道。致病力研究结果表明，渐狭蜡蚧菌JL003对二化螟3龄和6龄幼虫的致死率为53.0%和46.0%，球孢白僵菌JL005对3龄和6龄幼虫的致死率为94.0%和93.0%。对低龄和高龄二化螟幼虫，菌株JL005对二化螟的致死率均明显好于菌株JL003，表现出更大的生物防治潜能。
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| 关 键 词： | 二化螟 病原真菌 渐狭蜡蚧菌 球孢白僵菌 毒力测定 生物防治 |
| 收稿时间： | 2018-06-04 |
Pathogenic Fungi Identified from the Striped Stem Borer,Chilo suppressalis and Their Pathogenicity |
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| LI Meijun,BAI Qingrong,ZANG Liansheng,RUAN Changchun.Pathogenic Fungi Identified from the Striped Stem Borer,Chilo suppressalis and Their Pathogenicity[J].Chinese Journal of Biological Control,2019,35(1):63-69. |
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| Authors: | LI Meijun BAI Qingrong ZANG Liansheng RUAN Changchun |
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| Institution: | 1. Jilin Engineering Research Center of Biological Control/Institute of Biological Control, Jilin Agricultural University, Changchun 130118, China;2. College of Agronomy, Jilin Agricultural University, Changchun 130118, China |
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| Abstract: | Two strains JL003 and JL005 of pathogenic fungi were isolated from overwintering Chilo suppressalis larvae collected in Jilin province. The species were identified morphologically and using molecular systematics analysis of rDNA-ITS sequence. Meanwhile, the virulence of both strains against 3rd and 6th instars of C. suppressalis was determined using the dipping method. The strains JL003 and JL005 were identified to be Lecanicillium attenuatum and Beauveria bassiana, respectively, L. attenuatum being a first report from C. suppressalis in China. Pathogenicity study indicated the mortalities of C. suppressalis 3rd and 6th instars caused by L. attenuatum were 53.0% and 46.0%, respectively; those resulted from B. bassiana were 94.0% and 93.0%, respectively. Generally, B. bassiana exhibits high potential for biological control of C. suppressalis. |
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| Keywords: | Chilo suppressalis pathogenic fungi Lecanicillium attenuatum Beauveria bassiana toxicity bioassay biological control |
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