应用30个微卫星标记分析枫泾猪和小梅山猪的遗传多样性

为丰富枫泾猪与小梅山猪的种质特性,评价其保种效果,制订有效的保种方案,对枫泾猪与小梅山猪群体的遗传多样性进行分析,采用ISAG/FAO联合推荐的30个微卫星标记,分析2个猪群的遗传多样性。结果表明:枫泾猪的有效等位基因数(ne)、多态信息含量(PIC)、基因杂合度(He)、基因丰度(R)分别为(3.012 9±0.875 5)、(0.568 4±0.161 8)、(0.635 6±0.157 3)、(4.266 7±1.337 4),小梅山猪的遗传多样性参数中除平均观察等位基因数(na)与基因丰度外,其余均低于枫泾猪;2个群体间的遗传分化系数(Fst)与遗传距离(D)分别为0.005 3、0.008 7;枫泾猪与小梅山猪的遗传多样性较丰富,遗传分化程度小,均出现杂合子过剩现象,建议加强纯种繁育。

Genetic diversity analysis of Fengjing and Xiaomeishan pigs using 30 microsatellite markers

To enrich the information of the characteristic prosperities, evaluate the effect of breed conservation and make effective conservation measures for Fengjing and Xiaomeishan pigs, a total of 30 microsatellite markers co-recommended by the International Society of Animal Genetics (ISAG) and Food and Agriculture Organization of the United Nations (FAO) were used to analyze the genetic diversity of these two breeds. The results showed that genetic parameters ne, PIC, He, R for Fengjing pigs were (3.012 9±0.875 5), (0.568 4±0.161 8), (0.635 6±0.157 3), (4.266 7±1.337 4), respectively. All of the genetic diversity parameters of Xiaomeishan pigs except na and R were less than those of Fengjing pigs. Fst and D between the two breeds were 0.005 3 and 0.008 7, respectively. The results indicated that Fengjing and Xiaomeishan pigs showed rich genetic diversity, little genetic differentiation and heterozygote excess, indicating pure breeding should be strengthened in the future.