辐射白刺花遗传多样性的ISSR和RAPD比较分析

采用ISSR和RAPD标记对8个⁶⁰Co-γ辐射梯度诱变白刺花(Sophora viciifolia)进行遗传多样性分析。结果表明:20条ISSR和6条RAPD引物的PPL多态率分别为51.37%和21.03%,ISSR的Nei's指数和平均Shannon指数均大于RAPD;ISSR的标记指数(1.28)是RAPD(0.20)的6.4倍,表明ISSR具有较高的标记效率。Mantel检测表明,ISSR+RAPD标记与ISSR标记的结果显著相关(P<0.05)。综合分析表明白刺花用ISSR标记可检测出更高的多态性,更能准确反映白刺花材料间的遗传关系。

Comparison of Genetic Diversity of Irradiated Sophora viciifolia Using ISSR and RAPD Markers

The RAPA and ISSR markers were used in analyzing the genetic diversity of mutagenic Sophora viciifolia Franch with eight different ⁶⁰Co-γ-rays gradients. The results showed that the percentage of polymorphic bands of 20 ISSR primers and 6 RAPD primers were 51.27% and 21.03%, respectively. The Nei's gene heterozygosity and Shannon's information index of ISSR were greater than RAPD. For 8 materials, ISSRs got a MI value(1.28) that was 6.4 times higher than that of RAPDs(0.20), indicating that ISSRs had a higher MI efficiency. The Mantel test revealed that the correlation was significant between ISSR+RAPDs and ISSRs(r=0.9791, P<0.05). Comprehensive research showed that ISSRs could detect the higher polymorphism of Sophora viciifolia, and more accurately reflect the genetic relationship among Sophora viciifolia.