| 应用基因芯片方法检测几种肠道菌 |
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| 引用本文： | 徐晓静,文思远,韩毅. 应用基因芯片方法检测几种肠道菌[J]. 华北农学报, 2007, 22(1): 182-183 |
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| 作者姓名： | 徐晓静 文思远 韩毅 |
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| 作者单位： | 内蒙古农业大学,动物科学与医学学院,内蒙古,呼和浩特,010018;军事医学科学院放射医学研究所,北京,100850;内蒙古卫生检疫站,内蒙古,呼和浩特,010021 |
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| 摘 要： | 采用基因芯片技术,对几种常见的肠道菌进行检测和鉴定,包括检测靶基因的扩增、基因芯片的制备、杂交反应和杂交结果的检测与分析4个步骤。结果表明:应用基因芯片对涉及4个菌属的23株肠道菌在相同的条件下进行了杂交检测,得到菌属特异性的杂交图谱,从而达到对细菌进行检测鉴定的目的。制备的基因芯片能够同时检测沙门菌属、志贺菌属、葡萄球菌属、耶尔森氏菌属的细菌。
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| 关 键 词： | 基因芯片 肠道菌 肠道菌检测 |
| 文章编号： | 1000-7091（2006）06-0182-02 |
| 修稿时间： | 2006-11-10 |
Detection and Identification of Intestinal Bacteria by Oligonucleotide Array |
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| XU Xiao-jing,WEN Si-yuan,HAN Yi. Detection and Identification of Intestinal Bacteria by Oligonucleotide Array[J]. Acta Agriculturae Boreali-Sinica, 2007, 22(1): 182-183 |
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| Authors: | XU Xiao-jing WEN Si-yuan HAN Yi |
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| Affiliation: | 1. College of Animal Science and Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot 010018, China; 2. Institude of Radiation Medicine, Academy of Military Medical Sciences , Beijing 100850 , China; 3. Inner Mongolia Sanitation Quarantine Bureau, Huhhot 010021, China |
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| Abstract: | To detect the intestinal bacteria quickly and accurately,an experimental procedure was set up using the gene chip technology to detect and identify common intestinal bacteria.Target gene was amplified and hybridized with prepared gene chip.The results showed 23 strains of bacteria in pure culture belong to 4 genera were successfully discriminated under comparatively same condition and a series of specific hybridization maps corresponding to each kinds of bacteria were obtained.Salmonella spp,Shigella spp,Proteus spp,Yersinia enterocolitica,were detected and identified by the technology of gene chip. |
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| Keywords: | Gene chip Intestinal bacteria Detection of intestinal bacteria |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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