蛹虫草主要有效成分分析

[目的]进一步开发蛹虫草,满足人们对药品和滋补保健品的需求。[方法]通过用HPLC测定核苷类化合物和氨基酸,乙醇沉淀法测定虫草多糖,比色法测定虫草酸,SOD Assay Kit-WST试剂盒测定SOD酶酶活分析蛹虫草的主要有效成分。[结果]蛹虫草子实体中含有虫草素(3′-脱氧腺苷)、腺嘌呤、脱氧胸苷、尿嘧啶、腺苷、次黄嘌呤、鸟苷、尿苷等核苷类化合物,18种氨基酸,其中以谷氨酸、精氨酸、天冬氨酸、亮氨酸含量最高;甘露聚糖和葡萄糖含量分别为13.88和16.68 mg/g,虫草酸含量为17 mg/g,胞内SOD酶酶活为515.40 U/g。[结论]蛹虫草的主要有效成分为:核苷类化合物(虫草素、腺苷、鸟苷、尿苷、肌苷)、虫草酸、虫草多糖、氨基酸、SOD酶等。

Analysis on the Main Effective Constituents in Cordyceps militaris

[Objective] The aim was to further exploiting Cordyceps militaris so as to satisfy the demand for medicine and nourishing health products by people.[Method] The main effective components in C.militaris were analyzed through determining nucleosides and amino acid by HPLC,cordycepin polysaccharide by ethanol precipitation method,cordycepin acid by colorimetry and SOD activity by SOD Kit-WST reagent box.[Result] In C.militaris fruiting body,there were nucleosides including cordycepin(3′-deoxyadenosine), adenine,deoxythymidine,uracil,adenosine,hypoxanthine,guanosine and uridine,18 amino acids,among them the contents of glutamate,arginine,aspartate and leucine were highest;the contents of mannan and glucose were 13.88 and 16.68 mg/g resp.;the content of cordycepin acid was 17 mg/g and the SOD activity in cell was 515.40 U/g.[Conclusion] The main effective components in C.militaris were nucleosides(cordycepin,adenosine,guanosine,uridine and inosine),cordycepin acid,cordycepin polysaccharide,amino acid,SOD,etc.