Characterization of biotin-autotrophic isolates derived from naturally occurring auxotrophic race 2 isolates of <Emphasis Type="Italic">Fusarium oxysporum</Emphasis> f. sp. <Emphasis Type="Italic">lactucae</Emphasis> |
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Authors: | Norihito Yamauchi Mamoru Satou Jyuichi Shimazu Takashi Shirakawa Seizo Horiuchi |
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Institution: | (1) National Institute of Vegetable and Tea Science, Kannondai, Tsukuba 305-8666, Japan;(2) National Agriculture and Bio-oriented Research Organization, Tsukuba, Japan;(3) National Agricultural Research Center for Tohoku Region, Iwate, Japan;(4) Present address: Nanto Seed Corporation Ltd., Kashihara, Japan |
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Abstract: | Race 2 isolates of Fusarium oxysporum f. sp. lactucae have been recognized as biotin auxotrophs and consequently have restricted growth on Puhalla's minimal medium (MM), which
contains no biotin. Biotin-autotrophic isolates were raised from race 2 isolates through cultural mutation that grew as well
on MM as they did on MM supplemented with biotin. These autotrophs were identical to the parental isolates in pathogenicity
on race differential cultivars of lettuce (Patriot, Banchu Red Fire, and Costa Rica No. 4), and thus were designated as race
2. A vegetative compatibility test indicated that the autotrophic isolates fell into the same vegetative compatibility group
as the parents. Culture filtrates of the autotrophs allowed abundant growth of the parental auxotroph on MM, and, through
a competitive enzyme-binding assay, biotin was detected in the culture filtrates. These results suggest that biotin auxotrophy
in the natural race 2 isolates has no direct relation to pathogenicity, qualitatively defined as physiological race, or to
vegetative compatibility. |
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Keywords: | Fusarium oxysporum f sp lactucae Biotin-auxotrophy Race Pathogenicity Vegetative compatibility |
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