大蒜发芽叶愈伤组织细胞超微结构观察与过氧化物酶同工酶的变化

在大蒜发芽叶愈伤组织发生过程中,线粒体、内质网、核糖体和质体的数量呈增加趋势,结构呈完善趋势,细胞液泡化程度降低;而愈伤组织细胞中,这些细胞器的数量有所下降,有的线粒体发生解体.细胞脱分化过程中,细胞分裂以核缢裂的无丝分裂方式进行.在愈伤组织发生过程中,过氧化物酶同工酶谱呈减少和减弱的趋势,而在愈伤组织中,又有所增加和增强.

An Ultrastructural Study and Changes in Peroxidase Isozyme Patterns During Callus Induction from Garlic Sprout Leaf

In the process of callus induction from garlic sprout leaf, the amount of organelles as mitochondria, endoplasmic reticulum, ribosomes and plas-tid increased successively, their structures flourished gradually and the cells were with lower vacuolation. In the cell of callus, the amount of these organelles decreased, and some of mitochondria disintegrated. In the process of cell dedifferentiation, cell division form was the amitosis of the nucleus break-up.In the production of callus from garlic sprout leaf, the patterns and activity of the isoperoxidase existed a decreasing tendency, but a increasing and intensive tendency in the cell of callus.