一株产聚－β－羟基脂肪酸酯嗜盐菌的筛选与鉴定1）

以大庆地区盐碱土壤为样品经苏丹黑B染色、尼罗蓝染色分离出6株具有聚－β－羟基脂肪酸酯（ PHA）合成能力的菌株，选取产量较高的DQ－4进行鉴定，通过细菌形态学鉴定、16S rRNA分析并结合其生理生化特性，将菌株DQ－4鉴定为嗜盐单胞菌（ Halomonas sp．）。 GC－MS分析表明，菌株DQ－4合成的聚合物具有与PHA标样相似的峰值。 FT－IR分析显示，菌株DQ－4合成的聚合物在1726、2980、2934、2876 cm－1处出现了PHA的特征性吸收峰。因此，将其产物确定为PHA类化合物。菌株DQ－4的最适生长条件，温度38℃、pH值＝9、转速160 r· min－1、500 mL锥形瓶的装液量为100 mL，此条件下菌株DQ－4的PHA产量可达5．88g · L－1。

Isolait on and Identification of A PHA-accumulating Haol philic Bacterium and Its Growth Characteristics

Six PHA-accumulating strains were isolated with the usage of Sudan black stain , Nile blue staining from saline soil in the region of Daqing , China.Impressing on bacteria morphology salinity resistance , combined with the analysis of the 16S rRNA, a higher strain named DQ-4 was identified as Halomonas sp..The product of strain DQ-4 was similar with PHA standard by GC-MS.The FT-IR spectra of strain DQ-4 had a characteristic sharp absorption band around 1 726, 2 980, 2 934 and 2 876 cm-1 .The optimal growth conditions for strains of DQ-4 are 38℃temperature, pH value of 9, the speed of 160 r· min-1, and the fluid volume of 100/500 mL in flask with the PHA synthesis of 5.88 g· L-1 .