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丛枝菌根真菌对黄檗耐盐能力的影响
引用本文:赵匠,徐佳晶,李霞.丛枝菌根真菌对黄檗耐盐能力的影响[J].东北林业大学学报,2016(11):74-77.
作者姓名:赵匠  徐佳晶  李霞
作者单位:吉林师范大学,四平,136000
基金项目:国家自然科学基金项目(30271045)。
摘    要:采用温室盆栽试验,研究盐胁迫下,接种摩西球囊霉(Glomus mosseaw)和根内球囊霉(Glomus untraradices)对黄檗耐盐能力的影响。结果表明:盐胁迫下,丛枝菌根(AM)真菌对黄檗侵染率影响不显著(p0.05),黄檗叶片丙二醛(MDA)质量分数显著高于对照处理,接种AM真菌处理的黄檗叶片MDA质量摩尔浓度均显著低于不接种处理(p0.05);脯氨酸质量分数均较未接种植株升高,AM真菌有效地减轻黄檗受胁迫的程度。接种AM真菌显著增加了可溶性蛋白质量分数,AM真菌增强了黄檗非酶促防御系统能力。盐胁迫下,过氧化物酶(CAT)和过氧化氢酶(POD)活性在菌根和非菌根处理间差异不显著(p0.05),超氧岐化酶(SOD)活性在菌根和非菌根植株处理间差异显著(p0.05)。接种AM真菌后,菌根化黄檗植株可通过渗透调节物质积累和抗氧化酶活性的提高,降低体内膜脂过氧化产物的水平,缓解胁迫对黄檗植株的伤害,增强了黄檗对盐胁迫的耐受力。

关 键 词:丛枝菌根真菌  黄檗  盐胁迫

Effects of Arbuscular mycorrhizal Fungi on Salt Tolerance Ability of Phellodendron amurense
Abstract:The experiment was conducted to study the effect of inoculated with Glomus mosseaw and G.untraradices on salt toler-ance ability under salt stress by a pot experiment under greenhouse conditions .The effect of infection rate of Arbuscular my-corrhizal ( AM) fungi under salt stress on Phellodendron amurense has no significance ( p>0.05) .MDA content in leaves of P.amurense under salt stress was significantly higher than that of the control treatment , and the MDA content in leaves of P.amurense inoculated with two species fungi treatment was significantly lower than that of non -inoculation treatment ( p<0.05) .The proline content was higher than that of non inoculation treatment , AM fungi can effectively reduce the degree of stress of P.amurense.Inoculated treatment with two species fungi significantly enhanced soluble protein , and AM fungi can enhance non-enzymatic defensive System ability of P.amurense.There were not significantly difference mycorrhizal and non-mycorrhizal treatments of CAT and POD activities under salt stress (p>0.05), in addition, there was significantly dif-ferent between the mycorrhizal and non-mycorrhizal plants of the SOD activity ( p<0.05) .After inoculated with AM fungi , mycorrhizal plants of P.amurense can be, through osmoregulation substance accumulation and antioxidant enzyme activity , raised to reduce the level of lipid peroxidation productions , to relieve the stress on the plant damage of P.amurense, and to enhance the salt tolerance of P.amurense.
Keywords:Arbuscular mycorrhizal  Phellodendron amurense  Salt stress
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