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多倍体萱草的离体快繁技术
引用本文:尹立辉,武术杰,刘亚亮,温娜,王丽兰,王雪松. 多倍体萱草的离体快繁技术[J]. 东北林业大学学报, 2016, 0(8). DOI: 10.3969/j.issn.1000-5382.2016.08.008
作者姓名:尹立辉  武术杰  刘亚亮  温娜  王丽兰  王雪松
作者单位:1. 长春大学,长春,130012;2. 吉林省技秾种业有限公司;3. 中邦园林股份有限公司
基金项目:吉林省科学技术开发(委托)项目(2010220001000250)
摘    要:以多倍体萱草的嫩叶、生长点、腋芽、花蕾、花瓣和花茎作外植体进行离体快繁技术研究。结果表明:以幼嫩的花蕾和花瓣为外植体最为适宜;以MS+6-BA 0.5 mg/L+NAA 0.2 mg/L+2,4-D 0.7 mg/L为最适诱导培养基,诱导分化率可达74%以上;以MS+6-BA 2.0 mg/L+NAA 0.2 mg/L为最适继代增殖培养基,增殖系数可达5.8;以MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+IBA 0.02 mg/L+马铃薯20 g/L为壮苗培养基。培养1~2周后转入MS+NAA0.04 mg/L的生根培养基中,其平均根长、根长势等最为理想,生根率达100%,且移栽后成活率高,生长健壮。

关 键 词:多倍体萱草  花蕾  花瓣  离体快繁

Rapid in Vitro Propagation of Polyploid Hemerocallis fulva
Abstract:The experiment was conducted to study the rapid in vitro propagation of polyploidy Hemerocallis fulva with tender leaf , growing point, axillary bud, bud, petal and scape as explants .The young bud and petal were the most suitable explants . MS+6-BA 0.5 mg/L+NAA 0.2 mg/L+2,4-D 0.7 mg/L was the most suitable inducing medium , and the inducing differen-tiation rate was 74%.MS+6-BA 2.0 mg/L+NAA 0.2 mg/L was the most suitable subculture medium , and proliferation co-efficient was 5.8.MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+IBA 0.02 mg/L+Potato 20 g/L was the strong seedling medium . After 1-2 weeks, tissue culture seedling was transferred to the rooting medium of MS+NAA 0.04 mg/L.The average root length and root growth of tissue culture seedlings were the most ideal with the rooting rate of 100%, the survival rate was high, and the growth was strong .
Keywords:Polyploid Hemerocallis fulva  Bud  Petal  Rapid in vitro propagation
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