鹿茸角软骨细胞体外培养方法的建立及生长特性分析

【研究目的】建立梅花鹿鹿茸软骨细胞体外分离培养和扩增方法，观察鹿茸软骨细胞在体外培养的生物学特性，为鹿茸再生机理的研究奠定基础；【方法】体外分离培养鹿茸软骨细胞，采用组织学、MTT比色法、免疫组化等多种手段动态检测细胞生长增殖情况；【结果】核心部分，约150字鹿茸软骨细胞贴壁生长，原代细胞比传代生长速度快，原代及传代4代内的鹿茸软骨细胞均有活跃的增殖能力，软骨细胞呈三角形，多边形等多种形态，II型胶原免疫组化，甲苯胺蓝染色为阳性；【结论】体外分离培养鹿茸软骨细胞具有较强的增殖能力，传代培养4代内细胞生长旺盛并维持其生物学特性, 可满足后续实验研究要求。

Growth and Biological Character of Chondrocytes from Deer Antler in Vitro

Objective] To establish the in vitro culture and expense methods of sika deer antler chondrocytes for the investigation of antler regeneration study and analyze the biological character of cultured antler chondrocytes. Method] By the techniques of cell culture in vitro, the culture in vitro of the antler chondrocytes were performed. The cells growth was checked up by applying the ways of histology, MTT assay and immunohistochemistry. Results] The antler chondrocytes growth rapidly in plastic cell cultural board and chondrocytes l population demonstrates extensive proliferation and retains the capacity from original passage to passage 4. The original cells growth was faster than the passage. The antler chondrocytes were triangle, polygon and other shape. Immunochemistry results showed that the cultured cells were positive for collagen IIand . dDyeing of toluidine blue was positivet. Conclusion] The results showed that the chondrocytes of isolated antler presented proliferous ability in culture by our method. Chondrocytes can be cultured and extensively proliferate and retain biological feature for at least for 4 passages. The methods of antler chondrocytes culture can be used for the related continued research.