| 毛竹简易基因组文库的快速构建 |
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| 引用本文： | 梁银燕,周明兵,汤定钦. 毛竹简易基因组文库的快速构建[J]. 世界竹藤通讯, 2008, 6(2): 14-18 |
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| 作者姓名： | 梁银燕 周明兵 汤定钦 |
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| 作者单位： | 浙江林学院浙江省现代森林培育技术重点实验室,临安,311300;浙江林学院浙江省现代森林培育技术重点实验室,临安,311300;浙江林学院浙江省现代森林培育技术重点实验室,临安,311300 |
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| 摘 要： | 以毛竹（Phyllostachys edulis）当年所发新叶为材料，用改良的CTAB法提取基因组DNA，通过超声波震断DNA，琼脂糖凝胶电泳回收大小为700bp-2000bp的片段，经T4DNA聚合酶末端补平，与经过牛小肠碱性磷酸酶处理的pUCl9载体连接，通过电转化转染受体菌XL-1 Blue，构建了毛竹基因组文库。文库容量为2．8×10^7，通过PCR验证，插入片段大小为750 bp-2000bp，插入效率为91％，符合基因组文库构建的要求，为毛竹特异功能基因的克隆和分子标记的研究奠定了基础。
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| 关 键 词： | 毛竹 基因组文库 PCR |
Rapid Construction of Simple Genomic DNA Library from Leaves of Phyllostachys edulis |
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| Liang Yinyan,Zhou Mingbing,Tang Ding-Qin. Rapid Construction of Simple Genomic DNA Library from Leaves of Phyllostachys edulis[J]. World Bamboo and Rattan, 2008, 6(2): 14-18 |
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| Authors: | Liang Yinyan Zhou Mingbing Tang Ding-Qin |
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| Affiliation: | (Key Lab for Modern Silvicultural Technology of Zhejiang Province, Zhejiang Forestry University, LinAn 311300, ZheJiang) |
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| Abstract: | The genomic DNA was extracted from the young leaves of Phyllostachys edulis with modified CTAB method. After the DNA was sonicated, the DNA with the fragments ranging from 700 bp to 2 000 bp was ligased into pUC19 vector/Sma I＋CIP, which was transformed into XL-1 Blue by electroporation transformation. Results showed that the library reached 2.8×10^7 in capacity and contained over 91% recombinant bacteria, in which inserts fragments ranging between 750 bp and 2 000 bp in sizes through PCR. The successful construction of the cDNA library has laid a base for the study on characterization and cloning of specific genes and molecular markers of Phyllostachys edulis. |
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| Keywords: | Phyllostachys edulis genomic DNA library PCR |
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