The effect of different grading equipment on stress levels assessed by catecholamine measurements in Pacific oysters,Crassostrea gigas (Thunberg) |
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| Authors: | Yi Qu Xiaoxu Li Yi Yu Meegan Vandepeer Peter Babidge Steven Clarke Kriston Bott Hua Li |
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| Institution: | 1. Dalian Fisheries University, Heishijiao, Dalian 116023, PR China;2. South Australian Research and Development Institute, 2 Hamra Avenue, West Beach, SA 5024, Australia;1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;1. NIOZ – Royal Netherlands Institute for Sea Research, PO Box 59, 1790 AB Den Burg, Texel, The Netherlands;2. CIIMAR/CIMAR Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua dos Bragas 289, P 4050-123 Porto, Portuga;3. ICBAS – Instituto de Ciências Biomédicas Abel Salazar, Laboratorio de Fisiologia Aplicada, Universidade de Porto, Rua de Jorge Viterbo Ferreira n.° 228, 4050-313 Porto, Portugal;1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2. Key Laboratory of Mariculture & Stock Enhancement in North China''s Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China;3. Dalian Polytechnic University, Dalian 116034, China;4. University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | In this study the effects of exposure to two air temperatures (15 and 27.5 °C) and three graders commonly used in South Australia (Rotary, Flat Bed and Inside/Out) on Pacific oysters were investigated using post-exposure or post-grading catecholamine levels as a stress indicator. During the 48 h experimental period the circulating dopamine and noradrenaline levels of oysters exposed to 15 °C air temperature did not differ significantly from controls sampled directly from the 15 °C water tank. Conversely, in oysters exposed to 27.5 °C air temperature, dopamine and noradrenaline became significantly different from controls after 8 and 12 h air exposure, respectively, and then returned to the control levels at 24 h.Significant increases (P < 0.05) in circulating noradrenaline and dopamine levels were observed in oysters graded by the Flat Bed and the Inside/Out graders. The circulating noradrenaline concentrations in oysters subjected to the Flat Bed grader were significantly higher (P < 0.05) than those observed in the Rotary graded oysters and close to being significantly higher (P = 0.052) than those levels measured in the Inside/Out graded oysters. The circulating noradrenaline levels in oysters subjected to the Inside/Out grader were, in turn, significantly higher than those recorded in the Rotary graded oysters. These results suggest that in relation to the three graders used in South Australia, the Flat Bed induces the highest stress levels in oysters and the Rotary grader the lowest. |
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