Effect of stratification temperature,stratification period and seed coat on seed germination of peach cultivar ‘Sharbati’

Freshly harvested seeds of peach cultivar ‘Sharbati’ were found to be dormant and did not germinate at all. A specific low-temperature stratification treatment was required to overcome seed dormancy. 10° C stratification was found to be the best for breaking seed dormancy. Increased seed germination was recorded when the seeds were after-ripened without seed coats as compared to the seeds after-ripened with seed coats. There was no further increase in germination when seeds without seed coats were stratified beyond 60 days at 10° C, while a significant increase was recorded up to 75 days of stratification in the case of seeds with seed coats. Unstratified seed coats, soaked in water for 72 h, leached out a water-soluble inhibitor, which could suppress the germination of stratified peach seeds without seed coats. When this leachate was bioassayed by a cress-seed germination test, it showed the presence of an inhibitor at Rf 0.7-0.9. Hence, delayed germination of seeds with seed coats may be due to the presence of an inhibitor in seed coats.