油菜中转基因成分的筛查检测策略

旨在建立油菜中转基因成分的快速筛查方法,服务于农业转基因生物安全监管。根据转基因油菜常用转化遗传元件信息建立转基因油菜的筛查策略,并使用定性PCR法检测。结果表明,利用CaMV35S启动子、NOS终止子、bar基因、pat基因、CP4-epsps基因和NPT II基因6个靶标元件的组合,理论上可筛查92%的已知商业化转基因油菜转化事件。该方法的检测灵敏度达到1g/kg,可对油菜中的大多数转化事件进行快速、高灵敏度的筛查。

Screening Strategy of Genetically Modified Rapeseed Based on Inserted Genetic Elements

To establish a rapid screening method for genetically modified (GM) rapeseed and apply in the safety supervision of agricultural transgenic organisms,we developed a screening strategy and tested by using qualitative PCR method in this study based on the inserted genetic elements of commercial GM rapeseed.The results showed that combination of CaMV35S promoter,NOS terminator,bar gene,pat gene, CP4-epsps gene and NPT II gene as screening target elements could screen 92% of commercial genetically modified (GM) rapeseed,and the detection sensitivity was 1 g/kg.In conclusion,the screening strategy was a very valuable tool which it could quickly screen out GM rapeseed detection with high sensitivity and specificity.