Conditions for the induction of some selective enzymes from Bacillus subtilis and their hydrolysis ability against mackerel and gracilar (asparagus, Gracilaria verrucosa) |
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Authors: | Li -Jung Yin Pei -Chien Wu Hsiu -Ho Cheung Shann -Tzong Jiang |
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Affiliation: | Department of Sea Food Science, National Kaohsiung Marine University, Nan-Tzu, Kaohsiung,;National Taiwan Ocean University, Keelung, and;Department of Food and Nutrition, Providence University, Shalu, Taichung County, Taiwan |
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Abstract: | ABSTRACT: This study was conducted to optimize the cultivation conditions for Bacillus subtilis to produce proteases, amylases and cellulase, and to further investigate the hydrolysis ability against mackerel and asparagus. The extracellular enzymes from B. subtilis after 2 and 4 days incubation in a modified medium, containing 1% skim milk, 1% soya meal, 0.25% starch, 0.25% K2HPO4, 0.5% NaCl and 0.05% MgCl2 were collected for the hydrolysis of asparagus and minced mackerel, respectively. Except for the α,α-diphenyl-β-picrylhydrazyl (DPPH) scavenging ability of the hydrolyzed asparagus, the trolox equivalent antioxidation capacity and DPPH scavenging ability of both samples increased significantly ( P < 0.05) after 1 h hydrolysis and further increased during elongated hydrolysis at 50°C. Sodium dodecylsulfate–polyacrylamide gel electrophoresis indicated severe degradation of muscle proteins during hydrolysis. Changes in reducing sugar, soluble proteins and peptides before/after hydrolysis suggested the extracellular enzymes from B. subtilis could effectively hydrolyze the mackerel or asparagus, and subsequently improve their antioxidation ability. |
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Keywords: | antioxidation ability enzymes from Bacillus subtilis mackerel hydrolysate seaweed hydrolysate |
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