| 沙门氏菌invH基因的序列分析与分子检测 |
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| 引用本文： | 王俊红,刘高生,牛钟相,张文娟,裴宗飞.沙门氏菌invH基因的序列分析与分子检测[J].江西农业大学学报,2008,30(2):324-327. |
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| 作者姓名： | 王俊红 刘高生 牛钟相 张文娟 裴宗飞 |
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| 作者单位： | 1. 山东农业大学,动物科技学院,山东,泰安,271018
2. 聊城大学,农学院,山东,聊城,271000 |
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| 基金项目： | 山东省财政支持项目(SDGP-2004-54-0) |
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| 摘 要： | 根据沙门氏菌的invH基因序列设计1对引物,应用聚合酶链反应技术,分别对3种沙门氏菌标准菌株和5种非沙门氏菌株进行PCR扩增,结果3种标准沙门氏菌株均扩增出479 bp的特异条带,非沙门氏菌皆无特异带扩增。对3种标准株的扩增片段进行克隆及序列分析,证实沙门氏菌的invH基因序列比较保守;对19种地方分离沙门氏菌进行PCR特异性检测,结果有16株扩出特异性条带,表明本研究建立的沙门氏菌检测方法具有较高的特异性。
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| 关 键 词： | 沙门氏菌 invH基因 克隆 分子检测 |
| 文章编号： | 1000-2286(2008)02-0324-04 |
| 修稿时间： | 2007年11月28 |
DNA Sequenee Analysis and Molecular Detection of invH Gene from Salmonella spp. |
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| WANG Jun-hong,LIU Gao-sheng,Niu Zhong-xiang,ZHANG Wen-juan,PEI Zong-fei.DNA Sequenee Analysis and Molecular Detection of invH Gene from Salmonella spp.[J].Acta Agriculturae Universitis Jiangxiensis,2008,30(2):324-327. |
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| Authors: | WANG Jun-hong LIU Gao-sheng Niu Zhong-xiang ZHANG Wen-juan PEI Zong-fei |
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| Abstract: | A pair of oligonucleotide primers were synthesized according to the invH gene nucleotide sequence of Salmonella spp.and the invH gene was amplified from 3 strains of Salmonella and 5 strains of no-salmonella bacteria by PCR.The results showed that 3 strains of Salmonella yielded the 479 bp specific amplicon,but no specific product yielded in no-salmonella bacteria.The PCR products of 3 Salmonella spp.were cloned and sequenced.The result showed that the DNA sequences of invH gene from Salmonella spp.were consistent with the previous report.The invH gene-based PCR method for detecting Salmonella spp.was used to detect Salmonella spp.of 19 districts.16 specific strains were amplified.It is concluded that the invH gene-based PCR method is highly specific. |
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| Keywords: | Salmonella invH gene clone molecular detection |
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