| 六个灰树花菌株遗传多样性分析 |
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| 引用本文： | 王守现,刘宇,张英春,耿小丽,王兰青,孟莉莉. 六个灰树花菌株遗传多样性分析[J]. 北方园艺, 2010, 0(1) |
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| 作者姓名： | 王守现 刘宇 张英春 耿小丽 王兰青 孟莉莉 |
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| 作者单位： | 北京市农林科学院,植保环保研究所,北京,100097 |
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| 基金项目： | 国家科技支撑计划资助项目(2007BAD89B07) |
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| 摘 要： | 以6个灰树花菌株(灰分、灰通、灰怀、灰1、灰4和灰高)为材料,利用酯酶同工酶、RAPD和ISSR 3种分子生物学技术对其进行比较分析。结果表明:经酯酶同工酶经聚类分析,可将6个灰树花菌株分为三大类,灰分、灰通、灰怀和灰1为一类,灰4单独为一类,灰高单独为一类;经特异性较好的RAPD和ISSR引物验证,其结果与酯酶同工酶分析的一致。
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| 关 键 词： | 灰树花 酯酶同工酶 RAPD ISSR |
The Analysis of Genetic Diversity of Six Grifola frondosa Strains |
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| WANG Shou-xian,LIU Yu,ZHANG Ying-chun,GENG Xiao-li,WANG Lan-qing,MENG Li-li. The Analysis of Genetic Diversity of Six Grifola frondosa Strains[J]. Northern Horticulture, 2010, 0(1) |
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| Authors: | WANG Shou-xian LIU Yu ZHANG Ying-chun GENG Xiao-li WANG Lan-qing MENG Li-li |
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| Affiliation: | Institute of Plant Protection and Environment Protection;Beijing Academy of Agriculture and Forestry Sciences;Beijing 100097 |
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| Abstract: | Three molecular biology techniques of esterase isozyme electrophoresis,random amplified polymorphic DNA(RAPD) and inter-simple sequence repeat(ISSR)were used to analyze the genetic diversity of six Grifola frondosa strains.The results from the different bands analysis showed that the six Grifola frondosa strains were divided into three groups.Strains Huifen,Huitong,Huihuai and Hui1 were divided into one group.The strain hui4 and huigao was divided into one group,respectively.After being tested by special pr... |
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| Keywords: | grifola frondosa esterase isozyme electrophoresis RAPD ISSR |
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