| 2种提取猪肝基因组DNA方法的比较 |
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| 引用本文： | 周春丽,刘华,李玉萍. 2种提取猪肝基因组DNA方法的比较[J]. 安徽农业科学, 2009, 37(18): 8355-8356 |
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| 作者姓名： | 周春丽 刘华 李玉萍 |
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| 作者单位： | 江西科技师范学院生命科学学院,江西南昌,330013 |
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| 基金项目： | 江西科技师范学院院级项目，江西省教育厅科技项目，江西科技师范学院博士科研启动基金 |
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| 摘 要： | [目的]对2种猪肝基因组DNA提取方法，进行比较研究。[方法]分别采用试剂盒法和常规的氯仿／异戊醇抽提法，提取新鲜猪肝基因组DNA，并对其进行综合分析。[结果]试剂盒方法得到的DNA浓度较低，其OD260nm／OD280nm比值达到2.05，虽然蛋白质除的较干净，但是含RNA较高；而氯仿／异戊醇抽提法得到的DNA浓度高，其OD260nm／OD280nm比值1.88，纯度较高，且成本低。[结论]氯仿／异戊醇法对猪肝基因组DNA提取效果优于试剂盒法。
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| 关 键 词： | 猪肝 基因组DNA 氯仿/异戊醇抽提法 试剂盒法 比较 |
Comparison of Two Methods of Genomic DNA Extraction from Pork Liver |
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| ZHOU Chun-li et al. Comparison of Two Methods of Genomic DNA Extraction from Pork Liver[J]. Journal of Anhui Agricultural Sciences, 2009, 37(18): 8355-8356 |
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| Authors: | ZHOU Chun-li et al |
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| Affiliation: | ZHOU Chun-li et al(School of Life Science,Jiangxi Science & Technology Normal University,Nanchang,Jiangxi 330013) |
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| Abstract: | [Objective] The research aimed to compare the two extraction methods of genomic DNA from pork liver.[Method]kit method and chloroform/ isoamyl alcohol extraction method were adopted to extract genomic DNA from fresh pork liver.[Result] The results showed that the DNA extraction kit could obtain high purity genomic DNA with low density,and high concentration,good quality and relatively low cost by chloroform/ isoamyl alcohol method.[Conclusion] The chloroform/ isoamyl alcohol extraction of genomic DNA is a m... |
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| Keywords: | Pork liver Genomic DNA The chloroform/ isoamyl alcohol extraction kit method Comparison |
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