| 一株发酵乳杆菌培养条件的优化 |
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| 引用本文： | 洪梅,刁其玉,闫贵龙,屠焰,张乃锋,姜成钢.一株发酵乳杆菌培养条件的优化[J].饲料工业,2010,31(19). |
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| 作者姓名： | 洪梅 刁其玉 闫贵龙 屠焰 张乃锋 姜成钢 |
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| 作者单位： | 1. 中国农业科学院饲料研究所,100081,北京
2. 河北北方学院 |
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| 基金项目： | 国家"十一五"科技支撑项目"优质青贮和半干青贮技术研究及微生物添加剂开发" |
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| 摘 要： | 为更好地提高青贮饲料的质量,研究对分离自中国青贮窖中的一株乳酸菌BLF01进行了分子学鉴定,结果显示为发酵乳杆菌。然后通过单因素试验设计研究了培养基组成(碳源、氮源)和培养条件(温度、接种量、起始pH值)对BLF01乳酸菌生长繁殖的影响。结果表明,其最适碳源是乳糖,最佳氮源是酵母粉;最佳培养条件是起始pH值6.5、培养温度35℃、接种量1%,可以考虑将BLF01作为青贮接种剂使用。
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| 关 键 词： | 青贮饲料 发酵乳杆菌 培养基优化 |
Optimization of growth conditions for lactobacillus fermentum |
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| Hong Mei,Diao Qiyu,Yan Guilong,Tu Yan,Zhang Naifeng,Jiang Chenggang.Optimization of growth conditions for lactobacillus fermentum[J].Feed Industry,2010,31(19). |
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| Authors: | Hong Mei Diao Qiyu Yan Guilong Tu Yan Zhang Naifeng Jiang Chenggang |
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| Institution: | Hong Mei,Diao Qiyu,Yan Guilong,Tu Yan,Zhang Naifeng,Jiang Chenggang |
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| Abstract: | To improve the quality of silage and to decrease costs of microbial inoculants,culture conditions were optimized for the growth of a strain BLF01 of lactobacillus fermentum. The critical medium conditions including carbon source,nitrogen source,initial pH,temperature and inoculum size were identified by the one-factor-at-a-time approach. The optimal carbon source and nitrogen source were found to be lactose and yeast powder,respectively. The optimal pH,temperature and inoculum size for culture of BLF01 were... |
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| Keywords: | lactobacillus fermentum culture medium optimization |
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