| Direct somatic embryogenesis from leaves,cotyledons and hypocotyls of Hippophae rhamnoides |
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| 引用本文： | LIU Cui-qiong~1 XIA Xin-li~1 YIN Wei-lun~1 ZHOU Jiang-hong~2 TANG Hao-ru~3(1 College of Biological Sciences and Biotechnology,Beijing Forestry University 2 Phytopathology Key Laboratory,Yunnan Agricultural University 3 College of Forest and Horticulture,Sichuan Agricultural University). Direct somatic embryogenesis from leaves,cotyledons and hypocotyls of Hippophae rhamnoides[J]. 北京林业大学学报, 2006, 0(Z2) |
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| 作者姓名： | LIU Cui-qiong~1 XIA Xin-li~1 YIN Wei-lun~1 ZHOU Jiang-hong~2 TANG Hao-ru~3(1 College of Biological Sciences and Biotechnology Beijing Forestry University 2 Phytopathology Key Laboratory Yunnan Agricultural University 3 College of Forest and Horticulture Sichuan Agricultural University) |
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| 作者单位： | 1 College of Biological Sciences and Biotechnology，Beijing Forestry University 2 Phytopathology Key Laboratory，Yunnan Agricultural University 3 College of Forest and Horticulture，Sichuan Agricultural University |
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| 摘 要： | Direct somatic embryogenesis from leaves,cotyledons and hypocotyls of Hippophae rhamnoides
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Direct somatic embryogenesis from leaves,cotyledons and hypocotyls of Hippophae rhamnoides |
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| LIU Cui-qiong XIA Xin-li YIN Wei-lun ZHOU Jiang-hong TANG Hao-ru. Direct somatic embryogenesis from leaves,cotyledons and hypocotyls of Hippophae rhamnoides[J]. Journal of Beijing Forestry University, 2006, 0(Z2) |
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| Authors: | LIU Cui-qiong XIA Xin-li YIN Wei-lun ZHOU Jiang-hong TANG Hao-ru |
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| Affiliation: | LIU Cui-qiong~1 XIA Xin-li~1 YIN Wei-lun~1 ZHOU Jiang-hong~2 TANG Hao-ru~3 |
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| Abstract: | Plant regeneration via direct somatic embryogenesis from cotyledons, hypocotyls and leaves in Hippophae rhamnoides L. was achieved. The influence of basal media, carbon sources, plant growth regulators (PGRs) with different concentrations and combinations on embryogenesis capacity of explants was studied. The highest frequency of somatic embryo production and germination was obtained on Schenk and Hildebrandt medium (SH) supplemented with 1.0 mg/L kinetin (KIN) and 0.2, 0.5 mg/L indole-3-butyric acid (IAA). Granulated sugar was the optimal carbon source. The embryo-derived plantlets with well-developed roots and shoots were transferred successfully to the greenhouse with a maximum survival rate of 55%. Histological observation revealed that the somatic embryos were similar to those of zygotic embryos. |
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| Keywords: | plant regeneration Hippophae rhamnoides L. somatic embryogenesis |
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