| 胎猪胰腺干细胞体外诱导分化为胰岛素分泌细胞及其移植治疗裸鼠糖尿病的研究 |
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| 引用本文： | 楚元奎,曹晖,王赟,张翊华,窦忠英. 胎猪胰腺干细胞体外诱导分化为胰岛素分泌细胞及其移植治疗裸鼠糖尿病的研究[J]. 农业生物技术学报, 2011, 19(1). DOI: 10.3969/j.issn.1674-7968.2011.01.002 |
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| 作者姓名： | 楚元奎 曹晖 王赟 张翊华 窦忠英 |
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| 作者单位： | 西北农林科技大学国家干细胞研究中心陕西省分中心,杨凌,712100 |
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| 基金项目： | 国家高技术研究与发展计划(863)(No.2005AA219050); 国家自然科学基金(No.30671067)资助 |
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| 摘 要： | 缺乏供体细胞制约着胰岛替代疗法在Ⅰ型糖尿病治疗过程中的应用。本研究对实验室分离保存的胎猪胰腺干细胞(fetal porcine pancreatic stem cells,FPPSCs),体外诱导分化为胰岛素分泌细胞以及移植治疗裸鼠糖尿病的能力进行了深入研究,以探讨该细胞株作为异种供体细胞的潜力。研究结果表明,该细胞体外增殖活力旺盛,其形态及表面抗原表达特征与间充质干细胞极其相似,除表达胰腺干细胞相关标志外,FPPSCs还表达一些胚胎干细胞的相关标志。采用无血清诱导方案诱导2周后,FPPSCs形成DTZ染色阳性的胰岛样细胞团(islet-like cell cluster,ICC),该细胞团表达胰岛素、Glut-2等胰岛素分泌细胞功能相关的蛋白。RT-PCR结果显示,诱导后,胰腺干细胞相关标志表达减弱,而成熟胰岛素分泌细胞相关标志表达增强。葡萄糖刺激实验结果显示,诱导后,FPPSCs合成、分泌胰岛素和C-肽的能力显著增强(P<0.05)。将诱导2周后的细胞移植到糖尿病裸鼠腹腔内,可缓解其高血糖状况。结果提示,FPPSCs具有间充质干细胞的特性,体外能够诱导分化为胰岛素分泌细胞,并具有改善糖尿病裸鼠高血糖症状的...
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| 关 键 词： | 胎猪 胰腺干细胞 诱导分化 细胞移植 糖尿病 |
Fetal Porcine Pancreatic Stem Cells Differentiating into Insulin-producing Cells In vitro and Their Curative Effect on Diabetic Nude Mice |
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| Chu Yuankui,Cao Hui,Wang Yun,Zhang yihua,Dou Zhongying. Fetal Porcine Pancreatic Stem Cells Differentiating into Insulin-producing Cells In vitro and Their Curative Effect on Diabetic Nude Mice[J]. Journal of Agricultural Biotechnology, 2011, 19(1). DOI: 10.3969/j.issn.1674-7968.2011.01.002 |
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| Authors: | Chu Yuankui Cao Hui Wang Yun Zhang yihua Dou Zhongying |
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| Affiliation: | Chu Yuankui Cao Hui Wang Yun Zhang yihua Dou Zhongying Shaanxi Province Branch Center of National Stem Cell Engineering Techology Center,Northwest Sci-tech University of Agriculture and Forestry,Yangling 712100,China |
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| Abstract: | Islet replacement therapy is limited by the shortage of donor islet cells. The aim of this article is to study the potential capacity of fetal porcine pancreatic stem cells(FPPSCs) as a xenograft donor cells. FPPSCs have a strong proliferation ability,and the expression of it's surface antigen was similar to the mesenchymal stem cells. It expressed not only the markers of pancreatic stem cells,but also the markers of ES cells. After 2 weeks induction using a serum-free protocol,the DTZ-positive islet-like c... |
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| Keywords: | Fetal porcine Pancreatic stem cells Differentiation Cell transplantation Diabetes mellitus |
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