插核手术损伤对合浦珠母贝抗氧化免疫水平的影响

分析了插核手术(未插入珠核及小片)之后合浦珠母贝(Pinctada fucata)闭壳肌、鳃以及外套膜中超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性、总抗氧化能力(TAC)以及丙二醛(MDA)含量的变化,以探明合浦珠母贝抗氧化免疫系统对插核手术胁迫的响应特点。结果表明,各组织SOD、CAT活性和TAC基本上均呈现出下降-升高-再下降的波动变化。即大部分在第3小时达到最低水平而于第12小时达到或恢复到最高水平;相比之下,MDA含量整体上呈不断增高的趋势。相关性分析表明,MDA含量与TAC和CAT活性存在极显著的负相关性(P0.01);组织差异对SOD、CAT活性和TAC影响显著(P0.05),不同取样时间对SOD、CAT活性、TAC和MDA含量影响显著(P0.05)。研究结果显示插核手术损伤会造成合浦珠母贝抗氧化和免疫水平的波动,此过程中组织的氧化损伤程度与其抗氧化状态密切相关。

Effect of nucleus-inserting surgery damage on anti-oxidation and immunity of pearl oyster (Pinctada fucata)

We investigated the activities of superoxide dismutase (SOD) and catalase (CAT),total antioxidant capacity (TAC) and content of malondialdehyde (MDA) in the adductor,gill and mantle of pearl oyster (Pinctada fucata) after the pearl-nucleusinserting surgery (without nucleus and mantle piece),so as to study the effect of the surgery damage on anti-oxidation and immunity.The results show that the SOD,CAT activities and TAC in these three tissues experienced an increase-decrease-increase fluctuation after the surgery,and most of them reached the lowest values at the 3rd hour and the highest values at the 12th hour post-surgery.In contrast,the overall trend of MDA content was increasing during the experiment.Correlation analysis shows that CAT activity and TAC had significant negative correlation with MDA.Besides,significant difference was also detected in SOD,CAT activities and TAC among different tissues (P ＜ 0.01),as well as at different sampling time (P ＜ 0.01).The study suggests that the surgery damage may lead to fluctuation of anti-oxidation and immunity of P.fucata,and oxidant damage is closely related with the antioxidant status.