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| 鸡伤寒白痢沙门氏菌灭活菌苗的研制 | |
| 引用本文： | 成进,沙依然古丽,谷文喜,李文蓉,依米提,王泉.鸡伤寒白痢沙门氏菌灭活菌苗的研制[J].西北农业学报,2000,9(1):20-23. |
| 作者姓名： | 成进 沙依然古丽 谷文喜 李文蓉 依米提 王泉 |
| 作者单位： | 1. 新疆畜牧科学院兽医研究所 2. 新疆畜牧科学院农业部畜牧兽医生物技术重点开放实验室,乌鲁木齐,830000 3. 库尔勒市兽医站 |
| 基金项目： | 新疆维吾尔自治区科委重点科研项目 |
| 摘 要： | 由鸡沙门氏菌株C79-13和C79-20及新疆分离株AS97-19和AS96-4四株菌，经自制的改良硫代硫酸钠培养基，通气搅拌培养，甲醛灭活制成氢氧化铝佐剂苗，经免疫接种健康鸡，免疫后21d抗体水平达到最高，经ANAE方法和T淋巴细胞E花环方法检测，免疫组的细胞免疫水平明显高于非免疫对照组，差异极显著，10LD剂量强毒株攻菌试验，死亡保护率达94.7%，免疫组分离菌率为3.1%。表明该苗具有良好的安全性和免疫效果。 |
| 关 键 词： | 鸡伤寒沙门氏菌 鸡白痢沙门氏菌 鸡沙门氏菌灭活菌苗 |
| 文章编号： | 1004-1389(2000)01-0020-04 |
| 收稿时间： | 1999/6/8 0:00:00 |
| 修稿时间： | 1999-06-08 |
A Development of S.gallinarum and S.pullorum Inactivated Vaccine |
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| CHEN Jin,SAIERAN Guli,GU Wenxi,LI Wenrong,Yimitee and WANG Quan.A Development of S.gallinarum and S.pullorum Inactivated Vaccine[J].Acta Agriculturae Boreali-occidentalis Sinica,2000,9(1):20-23. | |
| Authors: | CHEN Jin SAIERAN Guli GU Wenxi LI Wenrong Yimitee and WANG Quan |
| Institution: | Institute of Veterinary, Xinjiang Acimal Science, Urumqi 83000;Institute of Veterinary, Xinjiang Acimal Science, Urumqi 83000;Institute of Veterinary, Xinjiang Acimal Science, Urumqi 83000;The Key Laboratory of Animal and Veterinary biotenology of MOA, Urumqi, Xinjiang 830000;Institute of Veterinary, Xinjiang Acimal Science, Urumqi 83000;Station of Veterinary, Kuerle Xinjiang |
| Abstract: | Four strains of S.avian C79-13,C79-20 and Xinjiang isolated strains AS97-19 and AS96-4 Were cultured by the heating stirculture in the improved sodium thisulphate medium and inactivated vaccine was prepared.The heal thy chickens were inoculated with the vaccine.The antibody against A.Salmonell a was detected on the 7th day and reached to the highest titer on the 21th day.Tly mphocytes was deter mined by ANAE and Eresottes methods.The results showed that the cellular immunity level of the immune group was obviously higher than the control group.When the chickens were challenged with 10LD strong strains,the protective rate was 94.7% and bacteriaisolated rate was 3.1%.The results indicated that the vaccine had good safety and immunity. |
| Keywords: | S gallinarum S pullorum Avian salmonella inactivated vaccine |
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