| 福建杉木王种质资源的遗传多样性 |
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| 引用本文： | 李明,黄木生,苏烁烁,陈细贞,马祥庆.福建杉木王种质资源的遗传多样性[J].福建林学院学报,2016(3):312-318. |
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| 作者姓名： | 李明 黄木生 苏烁烁 陈细贞 马祥庆 |
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| 作者单位： | 1. 福建农林大学林学院，福建 福州350002; 国家林业局杉木工程技术研究中心，福建 福州350002;2. 南平市林业局,福建 南平,353000;3. 福建农林大学林学院,福建 福州,350002 |
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| 基金项目： | 国家自然科学基金项目(U1405211)；福建农林大学林学院青年科学基金项目(6112C035006)。 |
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| 摘 要： | 为研究福建省杉木王种质资源的遗传多样性,在全面调查的基础上收集了福建省44份杉木王种质,并采用简单序列重复区间扩增多态性( ISSR)分子标记的方法分析其群体遗传多样性。结果表明,福建省杉木王主要分布在闽西、闽南和闽北地区,闽东地区分布较少。29条ISSR引物共扩增出237条带,平均每条引物扩增8.17条带；共有212条带具有多态性,多态性条带比率89％。福建省杉木王群体Nei′s基因多样性指数为0.3428, Shannon信息指数为0.5026,显示出较高的遗传多样性。非加权类平均法聚类分析和遗传结构分析均显示,福建杉木王可划分为5个类群,其类群划分结果与样本间的地理位置分布较为一致。
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| 关 键 词： | 杉木 遗传多样性 ISSR标记 种质资源 遗传结构 |
Genetic diversity of germplasm resources of the king of Chinese fir in Fujian provenances |
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| Abstract: | On the basis of comprehensive survey, 44 samples of the king of Chinese fir germplasm in Fujian provenances were collected, and the population genetic diversity was detected by inter-simple sequence repeats ( ISSR) molecular markers. The results showed that the king of Chinese fir were mainly distributed in western, southern and northern of Fujian, but rarely distributed in eastern. Twenty-nine primers were amplified into 237 bands, 8.17 bands on average from a primer amplification. Two hundred and twelve bands were polymorphic, accounting for 89%. The population Nei′s genetic diversity index was 0. 342 8, and the Shannon information index was 0.502 6, which revealed a relatively high genetic diversity. Both of the unweighted pair-group method with arithmetic means ( UPGMA) clustering and genetic structure analysis showed the king of Chinese fir in Fujian provenances could be divided into 5 groups, which were comparatively agree with the geography distribution. |
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| Keywords: | Chinese fir genetic diversity inter-simple sequence repeats marker germplasm resources genetic structure |
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