Analysis of genomic mutation and immunohistochemistry of platelet‐derived growth factor receptors in canine vascular tumours |
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| Authors: | S Abou Asa T Mori K Maruo A Khater A El‐sawak E Abd el‐Aziz T Yanai H Sakai |
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| Institution: | 1. Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Gifu University, Gifu, Japan;2. Department of Veterinary Pathology, Faculty of Veterinary Medicine, Kafr El‐Sheikh, Egypt;3. Laboratory of Clinical Oncology, Department of Veterinary Medicine, Gifu University, Gifu, Japan;4. Comparative Cancer Center, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan |
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| Abstract: | We examined whether mutation of the platelet‐derived growth factor receptor protein tyrosine kinase (PDGFR)‐α and PDGFR‐β genes contributes to their overexpression in canine vascular tumours. Genomic sequences of trans‐ or juxtamembrane regions of PDGFR‐α and PDGFR‐β were analysed with immunohistochemical staining and polymerase chain reaction‐direct sequencing using DNA from paraffin‐embedded neoplastic tissues of 27 hemangiosarcomas (HSAs) and 20 hemangiomas (HAs). Immunohistochemically, 75% of the HA cases were positive for PDGFR‐α and almost most of the HA cases were negative for PDGFR‐β. Of the HSA cases, 55.6% were negative for PDGFR‐α and 63% were strongly positive for PDGFR‐β. Among the HA cases, 1 missense mutation was detected in PDGFR‐α exon 18 and 1 in PDGFR‐β exon 17. Two HSA cases had missense mutations in exon 14 and 1 in exon 17 of PDGFR‐β. Thus, genomic mutation of trans‐ or juxtamembrane regions of PDGFRs was not the main mechanism driving the activation of receptors in HSA and HA. |
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| Keywords: | dog hemangioma hemangiosarcoma immunohistochemistry mutation PDGFR |
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