江华苦茶种质资源遗传多样性和亲缘关系的ISSR分析

利用ISSR标记分析了江华苦茶群体的遗传多样性和亲缘关系。10个多态性高、重复性好的ISSR引物在70个单株间共扩增出174条谱带,其中166条具有多态性,占95.40%,表明江华苦茶群体基因组DNA具有较高的多态性。供试单株间的平均Nei´s基因多态性（H）和Shannon信息指数（I）分别为0.382、0.558,表明材料间的遗传多样性较高。供试材料的相似系数介于0.44~0.84,平均为0.63。用UPGMA法进行聚类分析,共聚为6个类群,其中5个复合组,1个独立组。亲缘关系ISSR树状图在分子水平上显示了江华苦茶群体各单株间的亲缘关系,为江华苦茶种质资源的开发利用和保护提供了一定的参考依据。

Genetic Diversity and Relationship of Tea Germplasm Resources Camellia sinensis var.assamica cv.Jianghua Revealed by ISSR Markers

The 10 pairs of ISSR primers were used to analyze the genetic polymorphism and genetic relationship of 70 Camellia sinensis var.assamica cv.Jianghua individuals.Totally 174 bands were generated with 10 ISSR primers,of which 166(95.40%) were polymorphic,which indicated that the genetic diversity of Jianghua population was relatively high.The mean Nei′s gene diversity(H) and Shannon′s information index(I) of the tested tea germplasm were 0.382 and 0.558 respectively,indicating a rather high genetic diversity existed between the germplasm resources.The genetic similarity coefficient ranged from 0.44 to 0.84,averaging 0.63.UPGMA cluster analysis showed that all the tested individuals could be classified into 6 groups including 5 co mplex groups and 1 simple group.The genetic relationship of all 70 Jianghua individuals was revealed by the ISSR dendrogram,which provide the basis for the utilization and protection of this local landrace population.