| 利用RAPD分析大青杨天然群体的遗传结构 |
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| 引用本文： | 苏晓华 归复. 利用RAPD分析大青杨天然群体的遗传结构[J]. 林业科学, 1997, 33(6): 504-512 |
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| 作者姓名： | 苏晓华 归复 |
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| 作者单位： | 中国林业科学研究院林业研究所，北京市林业局 |
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| 基金项目： | 国家自然科学基金,劳动人事部非教育系统留学回国人员科技活动资助 |
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| 摘 要： | 本文利用ＲＡＰＤ分子标记技术从ＤＮＡ分子水平上探测了大青场（ｐｌｐｕｌｕｓｕｓｓｕｒｉｅｎｓｉｓＫｏｍ．）天然群体的遗传结构和分化程度。结果得出：用１４个随机寡核苷酸引物共产生１８０个扩增片段，扩增片断在２１１ｂｐ至１６３６ｂｐ之间。Ｓｈａｎｎｏｎ表型多样度（ＨＯ）估测值在群体间变动范围为０．２７１至０．３９２，平均为０．３１０。对分子水平变异分为群体间和群体内两部分进行分析，群体间分量占总变异的６２．３％，群体内只占３７．７％。不同引物在群体内探测能力也各不相同，ＣＨｌ－ｌ引物探测多样度（ＨＯ）最高（０．５４０），而２１１６引物最低（０．１５１）。
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| 关 键 词： | 大青杨，天然群体，RAPD，遗传结构 |
GENETIC STRUCYURE IN POPULUS USSURIENSIS KOM .CONFIRMED BY RAPD MARKERS |
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| Su Xiaohua Zhang Qiwen Zheng Xianwu Zhang Xianghua. GENETIC STRUCYURE IN POPULUS USSURIENSIS KOM .CONFIRMED BY RAPD MARKERS[J]. Scientia Silvae Sinicae, 1997, 33(6): 504-512 |
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| Authors: | Su Xiaohua Zhang Qiwen Zheng Xianwu Zhang Xianghua |
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| Abstract: | This paper described the population structure of P. ussuriensis Kom. The founeen random primers chosen for analysis generated 180 bands that ranged in size from 211 bp to 1636bp. Esdmates of Shannon's phenotypic divenity index nnged between 0.271 and 0.392, averaging 0.3 10. AnaJysis of molecular vadance parddoned the RAPD variation into the among-and within-population components. The among-population components accounted for 62.3% of the variation and withndpopuladon accounted for only 37.7yt. Es.mates of JVo also vuied among primers, with CH1-1, detecting . highest (0.540) and 2 11 6 detecting the lowest (0.151) Value Of diversitv. |
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| Keywords: | Populus Natural population RAPD Genetic sbucture. |
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