树脂—海藻酸钠混合凝胶共固定化糖化酶和酵母菌的研究

以D113离子交换树脂—海藻酸钠混合凝胶为载体,2%CaCl2溶液为固化液,采用包埋法共固定化糖化酶和酵母菌,并用于生料液态法白酒生产中。通过研究影响共固定化颗粒糖化发酵性能的因素,确定了共固定化的最佳条件:载体总量40mL,树脂浓度1.0%、海藻酸钠浓度3.5%、二氧化硅添加量为0.8%,糖化酶包埋量150mg,酵母菌包埋量60mg。将共固定化颗粒接入25%高粱粉发酵培养基中,30℃糖化发酵7d,酒度为15.0%。共固定化颗粒重复使用10次后,活力保存率为66.7%。

Co-immobilization of Glucoamylase and Yeast by Resin-sodium Alginate Gel

In this study,glucoamylase and yeast were co-immobilized onto the composite gel of resin D113 and sodium alginate by embedding method,with 2％ CaC12 solution as the solidified liquid.By analyzing the main factors in saccharifying fermentability,the optimal condition of co-immobilization were 1.0％ resin,3.5％ sodium alginate,0.8％silicon dioxide,150 mg glucoamylase,and 60 mg yeast,with the final volume of 40 ml.When co-immobilized glucoamylase and yeast were added into the fermentation medium with 25％ sorghum powder for 7 days at 30 ℃,the content of alcohol reached 15.0％,and the surplus vitality remained 66.7％,after 10 times usage.