春季动物园大熊猫粪便可培养真菌鉴定与分析

2011年2—5月，采集云南野生动物园3只亚成年雌性健康大熊猫的昼间新鲜粪便，经稀释、分离、纯化、保存，采用形态学观察、生化试验分析，对粪便可培养微生物进行真菌学鉴定与分析。结果表明，粪样分离得到霉菌和酵母菌2类真菌；霉菌分离鉴定有青霉属、木霉属、曲霉属和链格孢属；酵母菌分离得到红酵母菌属和德克酵母属；酵母菌检出率为83%，优势菌为红酵母菌属，为（531±062）×105个/g；粪样中酵母菌总数在早、中、晚各时段差异不显著（χ2=0933, P=0627＞005）；在个体间也不存在显著差异（H=1409，P=0494＞005）。

Identification and Analysis of Culturable Fungi in Spring Feces of Giant Panda(Ailuropoda melanoleuca) in Yunnan Wildlife Park

A study on the culturable fungi in the fresh feces of there sub-adult female giant pandas was conducted in Yunnan Wildlife Park during February to May of 2011.The culturable fungi isolated from the daytime fresh feces were identified and analyzed through dilution,isolation,purification,storage,morphological observation and bio-chemical experiment.The study found that yeast and mold fungi were isolated from the fecal samples.The mold fungi that were isolated and identified included genera Penicillium,Trichoderma,Aspergillus,and Alternaria,while the yeast fungi that was isolated from the feces sample included genera Rhodotorula and Dekkera.In addition,the detection rate of yeast fungi was 83%,in which Rhodotorula was the predominant genus,whose density was(5.31±0.62)× 105·g-1.There were no significant differences for the total number of yeast fungi among the samples collected at different periods of the daytime(χ2 = 0.933,P= 0.627> 0.05).There were either no significant differences for the total number of yeast fungi among the samples collected from the different animal individuals(H=1.409,P= 0.494> 0.05).