Occurrence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical primates from Brazilian Amazon |
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| Affiliation: | 1. Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal, SP, Brazil;2. Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal, SP, Brazil;1. Jaguar Conservation Fund/ Instituto Onça-Pintada, Caixa Postal 193, 75830-000, Mineiros-GO, Brazil;2. Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, 05508-000, São Paulo-SP, Brazil;3. Instituto de Biociências, Departamento de Parasitologia, Universidade Estadual Paulista, Campus de Botucatu, Distrito de Rubião Júnior, 18618-000, Botucatu-SP, Brazil;4. Instituto de Biologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, R. Ceará, s/n, 38400-902, Uberlândia-MG, Brazil;1. Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias/ Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil;2. Instituto Federal Goiano/ IF Goiano, Urutaí, GO, Brazil;1. Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz (UESC), Campus Soane Nazaré de Andrade Rodovia, Ilhéus Itabuna, Km 16, Salobrinho, Ilhéus, Bahia 45662-000, Brazil;2. Departamento de Patologia Animal, Universidade Estadual Julio Mesquita Filho, UNESP/Jaboticabal, Rod. Paulo Castelanne s/n, Jaboticabal, São Paulo 14884-900, Brazil;3. Departamento de Zootecnia, Universidade Estadual Julio Mesquita Filho, UNESP/Jaboticabal, Rod. Paulo Castelanne s/n, Jaboticabal, São Paulo 14884-900, Brazil;1. Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil;2. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres e Reservatórios, Fundação Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil;3. Instituto Nacional do Câncer (INCA), Rio de Janeiro, Brazil;1. Laboratório de Imunoparasitologia, Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil;2. Universidade Católica Dom Bosco, Campo Grande, MS, Brazil;3. Centro de Controle de Zoonoses (CCZ), Campo Grande, MS, Brazil |
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| Abstract: | Little is known about the prevalence and genetic diversity of Bartonella spp. and hemoplasmas in nonhuman primates (NHP). The present study aimed to investigate the occurrence of and assess the phylogenetic position of Bartonella spp. and hemoplasma species infecting neotropical NHP from Brazilian Amazon. From 2009 to 2013, a total of 98 blood samples from NHP belonging to the Family Cebidae were collected in the island of São Luís, state of Maranhão, of which 87 NHP were from Wild Animal Screening Center (CETAS) in the municipality of São Luís, and 11 (9 Sapajus sp. and 2 Saimiri sciureus) were from NHP caught in the Sítio Aguahy Private Reserve. DNA samples were screened by previously described PCR protocols for amplifying Bartonella spp. and Mycoplasma spp. based on nuoG, gltA and 16S rRNA genes, respectively. Purified amplicons were submitted to sequencing and phylogenetic analysis. Bacteremia with one or more Bartonella spp. was not detected in NHP. Conversely, 35 NHP were PCR positive to Mycoplasma spp. The Blastn analysis of seven positive randomly selected sequencing products showed percentage of identity ranging from 95% to 99% with other primates hemoplasmas. The Maximum Likelihood phylogenetic analysis based on a 1510 bp of 16S rRNA gene showed the presence of two distinct clusters, positioned within Mycoplasma haemofelis and Mycoplasma suis groups. The phylogenetic assessment suggests the presence of a novel hemoplasma species in NHP from the Brazilian Amazon. |
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| Keywords: | Hemotropic mycoplasmas Monkeys Amazon Brazil |
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