| 四种方法对组培室空气消毒效果的研究 |
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| 引用本文： | 邹瑜,林贵美,韦华芳,李朝生,牟海飞,LI Xiao-quan,ZHANG Jin-zhong,WU Dai-dong.四种方法对组培室空气消毒效果的研究[J].北方园艺,2009(8). |
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| 作者姓名： | 邹瑜 林贵美 韦华芳 李朝生 牟海飞 LI Xiao-quan ZHANG Jin-zhong WU Dai-dong |
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| 作者单位： | 1. 广西农业科学院,生物技术研究所,广西,南宁,530007
2. 广西植物组培苗有限公司,广西,南宁,530007 |
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| 基金项目： | 广西农业科学院科技发展基金资助项目(1999030);;广西“新世纪十百千人才工程”专项资金资助项目(2006225号) |
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| 摘 要： | 采用紫外线、空气灭菌器、艾叶+苍术、艾叶4种方法,对组培实验室进行空气消毒试验,分析其灭菌效果。结果表明:空气灭菌器0、.5 g/m3艾叶+2.0 g/m3苍术熏蒸消毒方法灭菌效果很好,灭菌率分别达82.7%和70.1%,达到了培养室含菌量低于50 cfu/m3的基本要求,在实际应用中,2种方法轮换使用效果最佳,组培污染率降低了50%~60%。使用中草药如艾叶、苍术等进行空气消毒灭菌,是植物组培生产中又一种新的空气灭菌方式。
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| 关 键 词： | 组培苗 空气消毒 污染 苍术 艾叶 |
Study on Air Disinfection of Tissue Culture Lab by Four Methods |
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| ZOU Yu,LIN Gui-mei,WEI Hua-fang,LI Chao-sheng,MOU Hai-fei,LI Xiao-quan,ZHANG Jin-zhong,WU Dai-dong.Study on Air Disinfection of Tissue Culture Lab by Four Methods[J].Northern Horticulture,2009(8). |
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| Authors: | ZOU Yu LIN Gui-mei WEI Hua-fang LI Chao-sheng MOU Hai-fei LI Xiao-quan ZHANG Jin-zhong WU Dai-dong |
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| Institution: | 1.Biological Technology Institute of Guangxi Academy of Agricultural Sciences;Nanning;Guangxi 530007;China;2.Guangxi Plant Tissue Culture Co.Ltd;China |
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| Abstract: | Four methods,ultraviolet radiation,air sterilizer,artemisia leaf,artemisia leaf with atractylodes were applied to sterilize the air of plant tissue culture lab.The result showed that two methods of air sterilizer,suffocating of 0.5 g/m3 artemisia leaf with 2.0 g/m3 atractylodes were preferable,and the rate of sterilize reach 82.7% and 70.1% respectively,fit for the standard of 50 cfu/m3 bacterium and fungi content of culture lab.The two methods can be applied in turn for sterilize of culture lab,and reduce ... |
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| Keywords: | Tissue culture plantlet Air disinfection Stain Atractylodes Artemisia leaf |
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