In Vitro Chemopreventive Potential of Phlorotannins-Rich Extract from Brown Algae by Inhibition of Benzo[a]pyrene-Induced P2X7 Activation and Toxic Effects |
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Authors: | Mlody Dutot Elodie Olivier Sophie Fouyet Romain Magny Karim Hammad Emmanuel Roulland Patrice Rat Roxane Fagon |
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Institution: | 1.Recherche & Développement, Yslab, 29000 Quimper, France;2.Faculté de Pharmacie de Paris, UMR CNRS 8038, Université de Paris, 75006 Paris, France; (E.O.); (S.F.); (R.M.); (K.H.); (E.R.); (P.R.) |
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Abstract: | Phlorotannins are polyphenols occurring exclusively in some species of brown algae, known for numerous biological activities, e.g., antioxidant, antiproliferative, antidiabetic, and antiallergic properties. Their effects on the response of human lung cells to benzoa]pyrene (Ba]P) has not been characterized. Our objective was to in vitro evaluate the effects of a phlorotannin-rich extract obtained from the brown algae Ascophyllum nodosum and Fucus vesiculosus on Ba]P cytotoxic effects. The A549 cell line was incubated with Ba]P for 48 and 72 h in the presence or absence of the brown algae extract. Cytochrome P450 activity, activation of P2X7 receptor, F-actin disorganization, and loss of E-cadherin expression were assessed using microplate cytometry and fluorescence microscopy. Relative to control, incubation with the brown algae extract was associated with lower Ba]P-induced CYP1 activity, lower P2X7 receptor activation, and lower reactive oxygen species production. The brown algae extract inhibited the alterations of F-actin arrangement and the downregulation of E-cadherin expression. We identified a phlorotannins-rich extract that could be deeper investigated as a cancer chemopreventive agent to block Ba]P-mediated carcinogenesis. |
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Keywords: | A549 cells cancer cytoskeleton phlorotannins P2X7 receptor pollution |
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