Methods for Selection of Low-Temperature Tolerant Mutants of Chrysanthemum morifolium Ramat. Using Irradiated Cell Suspension Cultures II. Preselection in vitro under Low-Temperature Stress |
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| Authors: | W Preil J B M Huitema J De Jong |
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| Institution: | Dr. W. Preil;, Federal Research Centre for Horticultural Plant Breeding, Bornkampsweg, W-2070 Ahrensburg (Germany) Dr. J. B. M. Huitema;, Agricultural University, Dept. of Plant Physiology, Arboretumlaan 4, NL-6703 BD Wageningen (The Netherlands) Dr. J. De;Jong, Centre for Plant Breeding Research (CPO), P.O. Box 16, NL-6700 AA Wageningen (The Netherlands). |
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| Abstract: | The effect of in vitro cold treatment (+6°C, 90 days) on selection of low-temperature tolerant (LTT) mutants was investigated using X-irradiated (15 Gy and 20 Gy) and non-irradiated cell suspensions of Chrysanthemum morifolium cv. ‘Parliament’. Low temperature reduced significantly the number of regenerating calli, especially in the irradiated cultures. After the end of the cold stress 684, 197 and 54 plants were obtained from the 0 Gy, 15 Gy and 20 Gy treated cultures, respectively. Testing these plants in the greenhouse (+12 °C, winter low-light) most of the early flowering variants were selected from the 15 Gy population. |
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| Keywords: | Chrysanthemum morifolium low-temperature tolerance cell suspension culture regeneration irradiation mutants |
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