| 利用RAP-PCR检测高盐刺激下Shewanella piezotolerans WP3的基因差异表达 |
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| 引用本文： | 李升康,李金媛,王玉桥.利用RAP-PCR检测高盐刺激下Shewanella piezotolerans WP3的基因差异表达[J].长江大学学报,2009(1). |
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| 作者姓名： | 李升康 李金媛 王玉桥 |
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| 作者单位： | [1]汕头大学理学院生物系,广东汕头515063 [2]国家海洋局生物遗传资源重点实验室,福建厦门361005 |
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| 基金项目： | 国家大洋开发协会项目(DY7000M-02) |
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| 摘 要： | 选取高盐刺激为条件，利用实验室模拟极端环境培养条件下的菌体提取RNA，采用差异显示方法RAP—PCR扫描盐刺激下基因的差异表达。结果表明，在高盐刺激下，WP3下调表达的基因是SSl：hypotheticalproteins SS2：ferroxidase。上调的基因是SS3：gamme-butyrobetaineantiporter；SS4：hypothetical protein。
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| 关 键 词： | Shewanella piezotolerans WP3 差异显示 高盐刺激 基因表达 |
Differential Expression of Shewanella piezotolerans WP3 upon High Salinity Stimulation Using RAP-PCR |
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| LI Sheng-kang College of Science,Shantou University,Shantou,Guangdong ,China,Key Laboratory of Marine Biogenetic Resources,Third Institute of Oceanography,Xiamen,Fujian ,ChinaLI Jin-yuan,WANG Yu-qiao Key Laboratory of Marine Biogenetic Resources,Third Institute ofOceanography.Differential Expression of Shewanella piezotolerans WP3 upon High Salinity Stimulation Using RAP-PCR[J].Journal of Yangtze University,2009(1). |
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| Authors: | LI Sheng-kang College of Science Shantou University Shantou Guangdong China Key Laboratory of Marine Biogenetic Resources Third Institute of Oceanography Xiamen Fujian ChinaLI Jin-yuan WANG Yu-qiao Key Laboratory of Marine Biogenetic Resources Third Institute ofOceanography |
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| Institution: | LI Sheng-kang College of Science,Shantou University,Shantou,Guangdong 515063,China,Key Laboratory of Marine Biogenetic Resources,Third Institute of Oceanography,Xiamen,Fujian 361005,ChinaLI Jin-yuan,WANG Yu-qiao Key Laboratory of Marine Biogenetic Resources,Third Institute ofOceanography |
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| Abstract: | The effect of the high salinity shock on the gene expression was performed in Shewanella piezotolerans WP3.The RNA bearing high salinity shock was isolated and then scanned by differential display RAP-PCR.The results showed that the genes encoding hypothetical protein and ferroxidase were induced and the genes anotated as gamme-butyrobetaine antiporter and hypothetical protein were repressed upon high salinity concentration stimulation. |
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| Keywords: | Shewanella piezotolerans WP3 differential display high salinity concentration stimulation gene expression |
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